Ionic Acid Demineralization for Bone Protein Release
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Solution Overview
Problem
The existing methods for producing osteoinductive compositions from demineralized bone matrix (DBM) are limited in their ability to effectively release native bone proteins, which are essential for bone formation and healing, due to the trapping of these proteins within the mineral component of bone tissue.
Innovation Solution
A method involving demineralization of bone tissue using an acid solution containing salt, specifically with concentrations between 0.5M to 10M, to expose native bone proteins, followed by optional processing into shaped materials like putty, gel, or blocks, to enhance the osteoinductive potential by increasing the concentration of bone proteins available for release.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If deionized water acid solution is used for demineralization, then the demineralization process is simple, but the bone protein concentration in the resulting composition is lower
Solution Approach 1:
The patent changes the ionic parameters of the demineralization solution by adding salts (NaCl, KCl, CaCl2, etc.) to achieve optimal protein release. The salt concentration is optimized at 0.5M to 10M, which significantly increases bone protein concentration in the resulting composition compared to deionized water-based solutions.
2Quantity of substance
If salt concentration is increased in the acid solution, then bone protein release is enhanced, but protein stability may be affected by ionic interactions
Solution Approach 1:
The patent optimizes salt concentration parameters to balance protein release and stability. The specific concentration range of 0.5M to 10M was determined to provide optimal conditions where ionic strength enhances protein extraction while maintaining protein conformational stability through controlled ionic interactions.
Solution Approach 2:
The patent replicates physiological ionic conditions found in natural bone environments by using salt solutions that mimic body fluid composition, thereby maintaining protein stability while enhancing release. This approach copies the natural conditions under which bone proteins function optimally.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method results in an osteoinductive composition with a higher concentration of native bone proteins, thereby accelerating bone formation and healing by optimizing the demineralization process, leading to improved bone grafting and repair applications.
Implementation Method 1
demineralizing the bone tissue to expose one or more native bone proteins, wherein demineralizing in step (c) comprises: (i) combining the bone tissue with an acid solution containing salt
Implementation Method 2
This demineralization leaves behind a collagen matrix and the various growth factors that may now be readily released in vivo
Implementation Method 3
Ionic content and concentration have complex effects on protein stability by modifying conformational stability and solubility. Ions binding to proteins may crosslink charged protein surfaces and lead to stabilization of the protein's native state
Implementation Method 4
The net effect of salt on protein stability is a balance of multiple mechanisms by which ions interact with protein molecules individually and protein-protein interactions
Data Source
AI summary
A method of producing an osteoinductive composition for in vivo use, in which the method includes obtaining bone tissue; cleaning the bone tissue with a plurality of washes; milling, grinding, and/or cutting the cleaned bone tissue into a plurality of bone pieces or fibers of a desired size; and demineralizing the plurality of bone pieces or fibers of a desired size to expose one or more native bone proteins, with demineralizing including combining the plurality of bone pieces or fibers in a reaction vessel with an ionic acid solution, soaking the plurality of bone pieces or fibers in the ionic acid solution, and exposing the one or more native proteins in the bone pieces or fibers.