Ionic Liquid Dispersive Microextraction for Metabolite Purification
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Solution Overview
Problem
Current sample preparation methods for biological samples are complex, require significant purification materials, and lack efficient miniaturization, making them inefficient for isolating and purifying analytical samples.
Innovation Solution
A method involving lysing biological samples with ionic liquids to denature intracellular metabolic enzymes, followed by mixing with organic solvents to form ionic liquid-organic solvent compositions, and then separating the ionic liquid to extract metabolites, using kits and systems for high-throughput analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If traditional sample preparation methods are used, then metabolites can be isolated, but the process is complex and requires significant purification materials
Solution Approach 1:
The patent extracts and removes intracellular metabolic enzymes through ionic liquid treatment, separating the harmful metabolic activity from the metabolites of interest. This extraction principle allows direct metabolite isolation without complex purification steps, reducing both material consumption and process complexity
Solution Approach 2:
The ionic liquid serves multiple functions simultaneously: it lyses cells, denatures enzymes to stop metabolism, and facilitates metabolite extraction. This multi-functionality consolidates multiple traditional preparation steps into one, reducing overall process complexity and material requirements
2Productivity
If traditional extraction methods are used, then metabolites can be purified, but the process is slow and inefficient
Solution Approach 1:
The ionic liquid is applied preliminarily to lyse cells and denature enzymes before metabolite extraction, preventing metabolic changes during preparation. This preliminary action stabilizes metabolite levels and eliminates the need for time-consuming post-extraction purification steps
Solution Approach 2:
The method skips traditional lengthy purification steps by using ionic liquid to directly extract metabolites from lysed cells. The process rushes through the critical enzyme denaturation and metabolite isolation phases in a single operation, dramatically reducing preparation time
3Reliability
If intracellular metabolic enzymes remain active, then cellular processes continue, but metabolite isolation is compromised
Solution Approach 1:
The patent converts the harmful effect of active metabolic enzymes (which alter metabolite levels) into a beneficial denaturation process. By using ionic liquid to denature enzymes, the method stops unwanted metabolism while simultaneously facilitating metabolite extraction, turning a problem into part of the solution
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method simplifies sample preparation by denaturing enzymes, reducing metabolic processes, and efficiently isolating metabolites with lower material usage, enabling faster and more efficient sample purification.
Implementation Method 1
contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample
Implementation Method 2
mixing the contacted cellular sample with an organic solvent to produce an ionic liquid-organic solvent composition
Data Source
AI summary
Provided herein is a method comprising one or more of the following steps: (a) lysing cells of a biological sample and contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample to produce a contacted cellular sample; (b) mixing the contacted cellular sample with an organic solvent to produce an ionic liquid-organic solvent composition; (c) mixing the contacted cellular sample with the organic solvent to produce a dispersed microdroplet ionic liquid-organic solvent composition; (d) contacting the ionic liquid-organic solvent composition with an ion exchange composition to produce a second ionic liquid-organic solvent composition; (d) separating the ionic liquid from the organic solvent; and (e) extracting metabolites from the ionic liquid. Kits and systems for practicing the subject methods are also provided.