Ionic Liquid Peptide Synthesis for High Yield
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Solution Overview
Problem
Current methods for producing peptides using ionic liquids result in low yields and are not suitable for industrial-scale production, as they often require protective groups, condensation agents, and are conducted at low reactant concentrations.
Innovation Solution
Converting a first amino acid or peptide into its ionic liquid form through an ionic bond, using it as both a reaction solvent and starting material, and reacting it with an ester of a second amino acid or peptide in the presence of water without peptide hydrolase or condensation agents to achieve high yield and concentration.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional peptide synthesis methods using ionic liquids are employed, then the reaction can be conducted in an ionic liquid medium, but the yield is low and the process is not suitable for industrial scale
Solution Approach 1:
The invention extracts and eliminates the harmful elements of conventional peptide synthesis by removing protective groups, condensation agents, and peptide hydrolases from the reaction system. This extraction of unnecessary components simplifies the manufacturing process and enables industrial-scale production while maintaining high peptide yields.
Solution Approach 2:
The invention changes the reaction parameters by conducting the reaction in an ionic liquid medium at high reactant concentrations without requiring protective groups or condensation agents. This parameter change transforms the reaction conditions to achieve both high yield and industrial scalability.
2Productivity
If protective groups and condensation agents are used in peptide synthesis, then the reaction can proceed in conventional conditions, but the process complexity increases and yield decreases
Solution Approach 1:
The invention extracts and eliminates the harmful elements of conventional peptide synthesis by removing protective groups, condensation agents, and peptide hydrolases from the reaction system. This extraction of unnecessary components simplifies the manufacturing process and enables industrial-scale production while maintaining high peptide yields.
Solution Approach 2:
The ionic liquid medium itself provides the necessary catalytic and solvation functions that previously required additional agents. The reaction system becomes self-sufficient, eliminating the need for separate protective groups and condensation agents, thereby reducing process complexity.
3Productivity
If reactant concentrations are kept low in ionic liquid reactions, then the reaction can be conducted smoothly, but the production efficiency and yield are reduced
Solution Approach 1:
The invention changes the reaction parameters by conducting the reaction in an ionic liquid medium at high reactant concentrations without requiring protective groups or condensation agents. This parameter change transforms the reaction conditions to achieve both high yield and industrial scalability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables the synthesis of peptides at high concentration and yield without the need for peptide hydrolase, simplifying the process and reducing production costs, making it suitable for industrial-scale production.
Implementation Method 1
Converting a first amino acid or peptide into its ionic liquid form through an ionic bond
Implementation Method 2
reacting it with an ester of second amino acid or peptide in the presence of water to form a peptide bond
Data Source
AI summary
Peptides may be produced by using (A) a first amino acid or peptide, which is converted into its ionic liquid form through the formation of an ionic bond, as a substance serving as both a reaction solvent and a reaction starting material; and reacting the first amino acid or peptide with (B) an ester of second amino acid or peptide, in the absence of any peptide hydrolase or any condensation agent, in the presence of water in an amount of not more than 20% by mass relative to the total mass of the reaction system to form a peptide bond between the first amino acid or peptide and the second amino acid or peptide. By means of this process, it is possible to synthesize a peptide at a high concentration and at a high yield, and this method is excellent for producing peptides on an industrial scale.