Ionic Surfactant Polymer for Immunoassay Interference Suppression
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing immunoassays face accuracy issues due to interfering substances in biological samples, which current methods struggle to address effectively, especially when measuring low-concentration substances, as they often require sample dilution that prolongs measurement time and may not account for unknown interfering substances.
Innovation Solution
Incorporating an ionic surfactant polymer with hydrophobic cyclic monomers and ionic functional groups into the immunoassay reaction solution to inhibit the influence of interfering substances, thereby enhancing measurement accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the test sample is preliminarily diluted to decrease the concentrations of interfering substances, then the accuracy of measurement is improved, but the time required for measurement is prolonged and the concentration of target substance is decreased
Solution Approach 1:
The patent introduces an ionic surfactant as an intermediary substance that binds to interfering substances in the sample matrix, preventing them from interfering with the immunoassay reaction. This mediator approach allows accurate measurement without requiring sample dilution, thus resolving the contradiction between measurement accuracy and measurement time.
2Measurement precision
If the test sample is preliminarily diluted to decrease the concentrations of interfering substances, then the accuracy of measurement is improved, but the concentration of target substance is decreased
Solution Approach 1:
The ionic surfactant acts as a mediator that selectively interacts with interfering substances while leaving the target substance unaffected. This allows the target substance to remain at its original concentration in the sample while the interfering substances are blocked, resolving the contradiction between maintaining target substance concentration and achieving accurate measurement.
3Measurement precision
If a compound is added to inhibit or suppress interfering activity for known substances, then the accuracy of measurement is improved, but the method is effective only for known specific substances and may not address unknown substances
Solution Approach 1:
The ionic surfactant employed in the patent has universal applicability across different sample types and interfering substances. Its amphiphilic structure and ionic characteristics enable it to bind to various types of interfering molecules (proteins, lipids, carbohydrates) regardless of their specific identity, providing broad-spectrum interference suppression that works for both known and unknown interfering substances.
4Object-affected harmful factors
If sample dilution is performed to reduce interfering substances, then the influence of interfering substances is reduced, but the measurement time is prolonged
Solution Approach 1:
The ionic surfactant serves as a mediator that binds to interfering substances in real-time during the immunoassay reaction, preventing them from affecting the measurement. This eliminates the need for pre-dilution steps, thereby reducing measurement time while maintaining the ability to suppress interfering substance influence.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The use of the ionic surfactant significantly reduces the impact of interfering substances, leading to more accurate immunoassay results with minimal effect on measurement time, even in the presence of unknown substances.
Implementation Method 1
an ionic surfactant having a molecular weight of 1000 to 100,000, the ionic surfactant being a polymer in which a hydrophobic cyclic monomer(s) having an ionic functional group(s) is(are) polymerized
Data Source
AI summary
Disclosed is an agent for inhibiting decrease in measured values in immunoassays, which may reduce the influences by interfering substances in a test sample so as to promote the accuracy of the immunoassays, as well as an immunoassay and a reagent for immunoassays using the same, with which the decrease in the measured values due to the interfering substances is reduced. The agent for inhibiting decrease in measured values in immunoassays, caused by interfering substances, is an ionic surfactant having a molecular weight of 1000 to 100,000, the agent being a polymer in which a hydrophobic cyclic monomer(s) having an ionic functional group(s) is(are) polymerized.