Iridium Complexes for Aqueous Electrochemiluminescence Detection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current electrochemiluminescence (ECL)-based detection methods, particularly for in vitro diagnostic assays, lack sensitivity and are limited by the use of water-soluble ruthenium complexes, which are not effective in aqueous media, necessitating the development of more sensitive labels for biological sample analysis.
Innovation Solution
The use of iridium-based Ir(III) luminescent complexes, specifically designed as chemiluminescent compounds and conjugates, which are suitable for ECL-based detection in aqueous solutions, enhancing sensitivity and efficiency in electrochemiluminescence reactions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If water-soluble ruthenium complexes are used for ECL-based detection, then the method is applicable to aqueous media, but the sensitivity is insufficient
Solution Approach 1:
The patent changes the metal center from ruthenium to iridium and modifies the ligand structure to achieve both high sensitivity and aqueous media effectiveness. The iridium complex with specific ligands (including carboxylic acid groups for aqueous solubility) resolves the contradiction by optimizing multiple parameters simultaneously.
Solution Approach 2:
The patent creates a composite iridium complex system combining the metal center with specifically designed ligands that have both luminescent properties and aqueous solubility characteristics. This composite approach allows the complex to function effectively in aqueous media while maintaining high detection sensitivity.
2Adaptability or versatility
If organic light emitting device compounds are used, then they are suitable for solid materials or organic fluids, but they are not effective in aqueous medium
Solution Approach 1:
The patent introduces hydrophilic groups (such as carboxylic acid groups) at specific positions on the ligand structure to provide aqueous solubility, while maintaining the core luminescent iridium complex structure. This local modification enables the compound to function in aqueous media without sacrificing its light-emitting properties.
Solution Approach 2:
The patent uses water-soluble ligands as intermediaries that bridge the iridium metal center to the aqueous environment. These ligands with hydrophilic groups act as mediators, allowing the hydrophobic iridium complex to interact effectively with aqueous media while maintaining its luminescent function.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The iridium-based chemiluminescent compounds provide high sensitivity and efficiency in ECL-based detection methods, enabling effective analyte measurement in aqueous solutions, overcoming the limitations of existing ruthenium complex-based systems.
Implementation Method 1
Electrogenerated chemiluminescence (also called electrochemiluminescence and abbreviated ECL) is the process whereby species generated at electrodes undergo high-energy electron-transfer reactions to form excited states that emit light.
Implementation Method 2
The present invention relates to novel iridium-based Ir(III) luminescent complexes
Data Source
AI summary
The present invention relates to novel iridium-based Ir(III) luminescent complexes, conjugates comprising these complexes as a label and their application, e.g. in the electrochemiluminescence based detection of an analyte.