ISG15 Protein Detection for Neurodegeneration Diagnosis
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Solution Overview
Problem
Current methods fail to effectively diagnose and address proteinopathies-induced neurodegeneration, which are chronic and incurable, lacking a common molecular mechanism for designing effective therapies.
Innovation Solution
A method involving the detection of ISG15 protein levels in samples, comparing them to control samples, to diagnose proteinopathy-induced neurodegeneration, using ISG15 bio-recognition elements like antibodies and kits that include autophagy and mitophagy markers for molecular signatures.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current diagnostic methods are used for neurodegenerative diseases, then diagnosis can be performed, but the methods fail to effectively diagnose and address proteinopathies-induced neurodegeneration
Solution Approach 1:
The patent changes the diagnostic parameter from general neurodegeneration markers to specific ISG15 protein levels and molecular signatures, enabling effective diagnosis of proteinopathy-induced neurodegeneration by measuring specific biochemical parameters rather than general clinical symptoms
Solution Approach 2:
The patent introduces ISG15 protein levels as an intermediary biomarker that mediates between the underlying proteinopathy mechanism and the observable neurodegeneration, allowing indirect but effective diagnosis of the disease process through a measurable molecular intermediate
2Ease of manufacture
If a common molecular mechanism is identified for proteinopathies, then effective therapies can be designed, but currently no common molecular mechanism is known
Solution Approach 1:
The patent extracts the common molecular mechanism information by measuring specific molecular signatures and ISG15 levels that are shared across different proteinopathies, separating the universal diagnostic features from disease-specific variations to enable general therapeutic approaches
Solution Approach 2:
The patent establishes a universal diagnostic approach using ISG15 measurement and molecular signature analysis that can be applied across multiple different proteinopathy types, creating a multi-functional diagnostic system that works for various neurodegenerative diseases with different underlying proteinopathies
3Reliability
If ISG15 detection methods are implemented, then patients at risk can be identified and treatment effectiveness assessed, but this requires new diagnostic tools and procedures
Solution Approach 1:
The patent replaces complex mechanical and clinical diagnostic procedures with a biochemical measurement system that detects ISG15 protein levels and molecular signatures, substituting physical examination and clinical observation with in vitro biochemical analysis
Solution Approach 2:
The patent creates a simplified diagnostic model by measuring ISG15 levels in accessible body fluids, creating a surrogate measurement system that copies the information about brain proteinopathy status from easily obtainable samples rather than requiring direct brain tissue analysis
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for the identification of patients at risk of proteinopathy-induced neurodegeneration and assesses treatment effectiveness by measuring ISG15 protein levels, providing a diagnostic tool for neurodegenerative diseases like Ataxia Telangiectasia and other proteinopathies.
Implementation Method 1
detecting the level of unconjugated ISG15 protein, conjugated ISG15 protein, or both in said sample
Data Source
AI summary
This invention is directed to compositions and methods for detecting proteinopathies.