Iso-Rebaudioside A Isomerization for Sweetness and Off-Taste Reduction

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Solution Overview

Problem

The food and beverage industry faces challenges in replacing high-calorie sweeteners with non-nutritive alternatives due to off-tastes such as slow onset, bitterness, or lingering aftertastes when using steviol glycosides like rebaudioside A, which limits their application in food and beverage products.

Innovation Solution

The development of steviol glycoside isomers, specifically iso-rebaudioside A, which is produced by heating rebaudioside A under acidic conditions, resulting in a compound with a new aglycone moiety and improved taste properties, allowing for its use as a potent non-nutritive sweetener in food and beverage products.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If steviol glycosides such as rebaudioside A are used as non-nutritive sweeteners, then sweetness potency is achieved, but off-tastes such as bitterness and lingering aftertaste occur

Engineering Contradiction:
Improvesweetness potencyVSAvoidoff-tastes including bitterness and lingering aftertaste
Core Design Contradiction:
Quantity of substanceVSObject-generated harmful factors

Solution Approach 1:

The patent applies parameter changes by modifying the chemical structure of steviol glycosides through isomerization. Specifically, the double bond position in the diterpene core is changed from exocyclic to endocyclic position, creating novel isomers with improved taste profiles while maintaining sweetness potency. This structural parameter change resolves the contradiction between achieving sweetness and avoiding off-tastes.

Inventive Principle:
Principle #35Parameter changes

2Loss of energy

If traditional steviol glycosides are used to replace high-calorie sweeteners, then calorie reduction is achieved, but taste quality deteriorates due to slow onset and off-tastes

Engineering Contradiction:
Improvecalorie contentVSAvoidslow onset and off-tastes
Core Design Contradiction:
Loss of energyVSObject-generated harmful factors

Solution Approach 1:

The patent modifies the molecular structure of steviol glycosides by isomerization to create novel compounds with improved sensory properties. The structural change in the diterpene core (double bond repositioning) enhances taste quality including onset speed and aftertaste profile, while maintaining the zero-calorie benefit, thus resolving the contradiction between calorie reduction and taste quality.

Inventive Principle:
Principle #35Parameter changes

3Quantity of substance

If known potent non-nutritive sweeteners are used, then sweetness intensity is improved, but off-tastes such as bitter and licorice aftertaste are generated

Engineering Contradiction:
Improvesweetness intensityVSAvoidbitter and licorice aftertaste
Core Design Contradiction:
Quantity of substanceVSObject-generated harmful factors

Solution Approach 1:

The patent employs parameter changes through chemical isomerization of the steviol glycoside structure. By repositioning the double bond from exocyclic to endocyclic position in the five-membered ring, the patent creates novel isomers that maintain high sweetness intensity while eliminating bitter and licorice aftertastes, thus resolving the contradiction between sweetness intensity and off-taste generation.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Iso-rebaudioside A offers enhanced sweetness potency and improved taste profiles, addressing the off-taste issues of traditional steviol glycosides, making it suitable for use in various food and beverage applications without significant caloric contribution.

Implementation Method 1

heating rebaudioside A under acidic conditions, resulting in a compound with a new aglycone moiety

Methodology Applied
Scientific EffectAcid-catalyzed isomerization:

Data Source

PatentEP2397485B1Steviol glycoside isomers
Publication Date: 2016.07.13 PEPSICO INC
  • EP2397485B1 patent drawingFigure 1
  • EP2397485B1 patent drawingFigure 2
  • EP2397485B1 patent drawingFigure 3

AI summary

Steviol glycoside isomers are provided having the formula: wherein R1 may be hydrogen, 1-β-D-glucopyranosyl, or 2-(1-β-D-glucopyranosyl)-1-β-D-glucopyranosyl, and R2 may be hydrogen, 1-β-D-glucopyranosyl, 2-(1-β-D-glucopyranosyl)-1-β-D-glucopyranosyl, 2,3-bis(1-β-D-glucopyranosyl)-1-β-D-glucopyranosyl, 2-(1-α-L-rhamnopyranosyl)-1-β-D-glucopyranosyl, 2-(1-α-L-rhamnopyranosyl)-3-(1-β-D-glucopyranosyl)-1-β-D-glucopyranosyl, or 2-(1-β-D-xylopyranosyl)-3-(1-β-D-glucopyranosyl)-1-β-D-glucopyranosyl. Methods for making steviol glycoside isomers are also disclosed. These compounds may be present in food and beverage products as non-nutritive sweeteners.