Integrated Isolation Chip for Rapid SARS-CoV-2 Detection
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Solution Overview
Problem
Current methods for detecting novel coronavirus (SARS-CoV-2) require separate devices for virus purification and detection, leading to inefficiencies and prolonged processing times.
Innovation Solution
An integrated isolation chip and detection device that combines particle isolation and detection using a reagent reservoir, filtration membranes, and a vacuum unit, along with enzyme-linked antibodies and chemiluminescent substrates to generate optical signals for target particle detection, allowing for on-chip detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If separate devices are used for virus purification and detection, then each device can be optimized for its specific function, but the overall processing time increases and efficiency decreases
Solution Approach 1:
The patent combines virus purification and detection functions into a single integrated chip device. The chip includes a reservoir for sample placement, filtration membranes for purification, and detection zones with antibodies for virus detection, all in one device. This merging eliminates the need to transfer samples between separate purification and detection devices, thereby reducing processing time while maintaining detection accuracy.
2Reliability
If multiple separate devices are used for virus detection, then each device can perform its specialized function, but the device complexity and operational steps increase
Solution Approach 1:
The integrated chip is designed to perform multiple functions within a single device: sample reception, virus purification through filtration membranes, virus concentration, and immunodetection using embedded antibodies. This multi-functional design reduces the number of separate devices needed while maintaining high detection sensitivity through specialized zones for each function.
3Reliability
If traditional purification methods are used before detection, then virus particles can be effectively purified, but the overall detection process becomes time consuming
Solution Approach 1:
The chip is segmented into distinct functional zones: a reservoir for sample placement, filtration membrane regions for purification, and detection zones with immobilized antibodies. This segmentation allows purification and detection to occur in different spatial regions simultaneously within the same device, improving both purification efficiency and detection speed by eliminating sequential processing bottlenecks.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Facilitates rapid and sensitive detection of target particles, reducing processing time to less than 15 minutes and improving detection sensitivity and accuracy by integrating isolation and detection processes in a single device.
Implementation Method 1
a filtration membrane in the second chamber
Implementation Method 2
a vacuum unit configured to alternately generate a negative pressure in the first chamber and the second chamber
Implementation Method 3
enzyme-linked antibodies and chemiluminescent substrates to generate optical signals for target particle detection
Implementation Method 4
enzyme-linked antibodies...combined with the purified target particles
Data Source
AI summary
An isolation chip for separating and isolating target particles from a bioliquid sample includes a reagent reservoir, a first filtration membrane, a second filtration membrane, a first chamber, and a second chamber. The reagent reservoir includes a first sidewall and a second sidewall opposite to the first sidewall. The first filtration membrane is disposed at an upper portion of the first sidewall. The reagent reservoir defines a first window at a lower portion of the first sidewall. The second filtration membrane is disposed at the second sidewall. The first chamber is connected to the reagent reservoir through the first filtration membrane. The second chamber is connected to the reagent reservoir through the second filtration membrane. A device and a method for detecting the target particles are further provided.