Nucleic acid isothermal amplification colloidal gold test device
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Solution Overview
Problem
Existing nucleic acid detection methods, such as PCR and qPCR, require complex instruments, multiple cycles of heating and cooling, and high costs, making them cumbersome and expensive for routine use.
Innovation Solution
A nucleic acid isothermal amplification colloidal gold test device with a modular design, utilizing a reagent reaction module and isothermal amplification technology, which allows for simplified operation without specialized equipment and reduced costs.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If PCR or qPCR methods are used for nucleic acid detection, then detection accuracy is improved, but device complexity and operational cost increase significantly
Solution Approach 1:
The patent extracts the core amplification function from complex PCR instruments and implements it through a simplified isothermal amplification system. The reaction module contains only essential components (reaction tube, heating element, reagents) without needing complex temperature cycling systems, fluorescence detectors, or data analysis software, thereby reducing device complexity while maintaining detection capability
Solution Approach 2:
The patent employs disposable reaction modules that are pre-filled with reagents and can be discarded after single use. This eliminates the need for expensive, complex instruments and extensive training, as each disposable module is self-contained and requires minimal operational complexity, resolving the contradiction between detection accuracy and device complexity
2Productivity
If PCR with multiple heating and cooling cycles is used, then amplification efficiency is improved, but reaction time and energy consumption increase
Solution Approach 1:
The patent changes the temperature parameter from cyclic variation (PCR) to constant isothermal condition. The isothermal amplification system maintains a single optimal temperature (e.g., 37°C or 42°C) throughout the reaction, eliminating the time-consuming heating and cooling cycles of PCR while maintaining high amplification efficiency through engineered enzymes that function optimally at constant temperature
Solution Approach 2:
The isothermal amplification enables continuous reaction at constant temperature without interruption for temperature cycling. The reaction proceeds continuously in a single direction (amplification) rather than cycling through denaturation, annealing, and extension phases, thereby reducing total reaction time while maintaining productivity
3Measurement precision
If fluorescent probes and qPCR instruments are used for quantitative detection, then measurement precision is improved, but operational cost increases
Solution Approach 1:
The patent uses inexpensive disposable reaction modules with visual readout (e.g., color change on test strips or turbidity) instead of expensive fluorescent probes and detectors. The results can be observed directly or with simple equipment, eliminating the need for costly qPCR instruments and specialized reagents, thereby reducing operational cost while maintaining sufficient measurement precision for diagnostic purposes
Solution Approach 2:
The patent replaces the optical detection system (fluorescence) with simpler visual or turbidity-based detection. Instead of using fluorescent probes that require expensive detectors and complex data analysis, the system uses colorimetric or turbidimetric changes that can be observed directly or with basic equipment, reducing operational cost while preserving quantitative detection capability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device achieves efficient and rapid nucleic acid amplification with visual results, reducing the need for complex instruments and training, and lowering operational costs while maintaining high reaction efficiency.
Implementation Method 1
the lower seat comprises a seat bottom and a lower seat shell, a heat-conducting plate is arranged in the seat bottom, the heat-conducting plate is provided with a heating plate, the lower seat shell is arranged on the heat-conducting plate, and the bottom of the solution box is in contact with the heating plate and the heat-conducting plate
Implementation Method 2
a heat-conducting plate is arranged in the seat bottom, the heat-conducting plate is provided with a heating plate, the lower seat shell is arranged on the heat-conducting plate, and the bottom of the solution box is in contact with the heating plate and the heat-conducting plate
Data Source
AI summary
The disclosure relates to the technical field of nucleic acid test, particularly to a nucleic acid isothermal amplification colloidal gold test device. The device comprises a lower seat and a reagent reaction module slideably arranged in the lower seat and comprising a reaction module shell, a reagent collection tube, a reagent reaction box, a solution box and a test paper installation assembly, the solution box is arranged at the bottom of the reaction module shell and located under the test paper installation assembly, the reagent reaction box is arranged on the solution box and partially located in the solution box, the reagent collection tube is arranged in the reaction module shell and the lower end is slideably matched with the reagent reaction box, and the test paper installation assembly is arranged in the reaction module shell and located outside the reagent collection tube.