JOLIET Bean Plant Genome Editing for Yield and Disease Resistance
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Solution Overview
Problem
There is a need for stable, high-yielding, and agronomically sound bean plant varieties that can maximize yield while maintaining desirable traits such as disease resistance, drought tolerance, and improved nutritional quality.
Innovation Solution
The development of the bean plant variety JOLIET, which includes methods for vegetative propagation, tissue culture, and introduction of desired traits through single locus conversion using genome editing techniques, such as CRISPR/Cas, to enhance characteristics like yield, disease resistance, and nutritional quality.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If traditional breeding methods are used to develop bean plant varieties, then genetic diversity is maintained, but the precision and speed of introducing specific desired traits is limited
Solution Approach 1:
The patent applies genome editing techniques (CRISPR/Cas, TALENs, zinc finger nucleases) to precisely modify specific genetic loci in bean plants, enabling direct introduction of desired traits without traditional multi-generational breeding. This changes the breeding parameter from gradual selection to targeted molecular editing, achieving both precision and time efficiency
Solution Approach 2:
The patent replaces traditional mechanical breeding methods (crossing, selection, and recombination) with molecular-level genetic editing tools. Instead of relying on random genetic recombination through crossing, the invention uses engineered nucleases to directly cut and modify DNA at specific locations, substituting a mechanical/biological system with a molecular engineering approach
2Productivity
If genome editing techniques are used to introduce desired traits, then breeding precision and speed are improved, but the complexity of the methodology increases
Solution Approach 1:
The patent employs a universal genome editing platform (CRISPR/Cas system) that can target multiple different genetic loci using the same core machinery. The guide RNA can be redesigned to target any desired gene, making the system multi-functional and adaptable to different breeding goals without requiring entirely different tools for each application
Solution Approach 2:
The patent uses guide RNA as an intermediary that directs the nuclease enzyme to specific DNA sequences. This intermediary component simplifies the targeting process by separating the cutting function (nuclease) from the targeting function (guide RNA), allowing independent optimization and reducing overall system complexity
3Manufacturing precision
If single locus conversion is used to introduce specific traits, then the precision of trait introduction is improved, but the adaptability to introduce multiple traits simultaneously is reduced
Solution Approach 1:
The patent divides the genome editing process into discrete, targetable loci. Each desired trait corresponds to a specific genetic locus that can be independently edited. This segmentation allows precise modification of individual traits while maintaining the ability to address multiple separate loci through sequential or simultaneous editing events
Solution Approach 2:
The patent combines multiple guide RNAs targeting different loci with a single nuclease enzyme to achieve simultaneous editing of multiple genetic locations. This merging approach allows introduction of multiple traits in a single transformation event, overcoming the limitation of single-locus conversion while maintaining precision
Data Source
AI summary
Novel bean plants, such as bean designated JOLIET are disclosed. In some embodiments, the disclosure relates to the seeds of bean plant JOLIET, to the plants and plant parts of bean JOLIET, and to methods for producing a bean plant by crossing the bean plant JOLIET with itself or another bean plant. The disclosure further relates to methods for producing other bean plants derived from the bean JOLIET.