Ju-Ling Compound Fingerprinting for Complex TCM Quality Evaluation
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Solution Overview
Problem
Current methods lack effective means for in-depth analysis of the impact of intrinsic efficacy components on the quality of the Ju-Ling herbal compound, a traditional Chinese medicine formulation for treating gout, and existing fingerprint construction methods for single TCM medicinal materials are not applicable to complex compounds like Ju-Ling herbal compound.
Innovation Solution
A method for constructing a fingerprint chromatogram of the Ju-Ling herbal compound using ultra-high performance liquid chromatography (UHPLC) with optimized chromatographic analysis conditions and selected reference substances, enabling comprehensive quality evaluation through similarity analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If traditional fingerprint construction methods for single TCM medicinal materials are used, then the method is simple and easy to operate, but it is not applicable to complex TCM compounds like Ju-Ling herbal compound
Solution Approach 1:
The patent develops a fingerprint construction method that can universally apply to both single TCM medicinal materials and complex TCM compounds. The method uses UHPLC technology with optimized chromatographic conditions that can handle diverse compound types, making the fingerprint construction approach multi-functional and broadly applicable across different TCM formulations.
Solution Approach 2:
The patent optimizes multiple chromatographic parameters including mobile phase composition (acetonitrile-water-formic acid system), gradient elution program, column temperature (30℃), and detection wavelength (300 nm) to achieve suitable separation for complex TCM compounds. These parameter adjustments enable the method to effectively resolve the complexity of multi-component TCM formulations.
2Measurement precision
If comprehensive quality evaluation of Ju-Ling herbal compound is achieved, then the accuracy and objectivity of quality assessment is improved, but the analysis method becomes more complex
Solution Approach 1:
The patent introduces a mixed reference substance solution containing multiple reference compounds (chlorogenic acid, gentiopicroside, swertiamarin, astilbin, rutin, quercitrin, narcissin) as intermediaries to facilitate comprehensive quality evaluation. These reference substances serve as markers to identify and quantify key components in the Ju-Ling herbal compound, enabling accurate and objective quality assessment through similarity analysis.
Solution Approach 2:
The patent replaces traditional complex multi-step analysis methods with UHPLC fingerprinting combined with similarity evaluation. This substitution uses advanced chromatographic separation and automated data processing to achieve comprehensive quality evaluation more efficiently, reducing manual intervention while maintaining or improving accuracy.
3Measurement precision
If UHPLC fingerprint chromatogram is constructed with optimized conditions, then the separation effect and detection accuracy is improved, but the time and resource consumption increases
Solution Approach 1:
The patent employs gradient elution with periodic changes in mobile phase composition over time (0-5 min: 5-10% acetonitrile; 5-20 min: 10-40% acetonitrile; 20-30 min: 40-60% acetonitrile; 30-35 min: 60-95% acetonitrile). This periodic adjustment of chromatographic conditions enables efficient separation of multiple components while controlling analysis time, achieving both high detection accuracy and reasonable time consumption.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method provides a comprehensive, objective, and accurate evaluation of the Ju-Ling herbal compound's quality, ensuring safety and efficacy by detecting and evaluating its complex composition effectively.
Implementation Method 1
separately injecting the test solution and the mixed reference substance solution into a high-performance liquid chromatograph, and performing similarity analysis on chromatograms obtained
Data Source
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AI summary
The present disclosure discloses a method for constructing a fingerprint chromatogram of a Ju-Ling herbal compound, including: (1) preparing the Ju-Ling herbal compound into a test solution; (2) preparing a mixed reference substance solution using chlorogenic acid, 6'-O-β-D-glucosylgentiopicroside, swertiamarin, gentiopicroside, sweroside, 3-O-caffeoylshikimic acid, neoastilbin, astilbin, rutin, isoastilbin, isochlorogenic acid C, quercitrin, kaempferol-3-O-rutinoside, and narcissin; and (3) separately injecting the test solution and the mixed reference substance solution into a high-performance liquid chromatograph, and performing similarity analysis on chromatograms obtained to obtain the fingerprint chromatogram of the Ju-Ling herbal compound. The present disclosure also provides the fingerprint chromatogram of the Ju-Ling herbal compound obtained by the method and its use in identifying and/or evaluating the quality of the Ju-Ling herbal compound. The fingerprint chromatogram of the Ju-Ling herbal compound according to the present disclosure could comprehensively, objectively, and accurately test and evaluate the quality of the Ju-Ling herbal compound, thereby ensuring the safety and efficacy of medication for patients.