Recombinant Keratin BD-3 Production via Host Cell Expression
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Solution Overview
Problem
Keratin, a crucial structural protein, is difficult to extract and utilize due to its insolubility in solvents and resistance to enzymatic hydrolysis, limiting its application despite its potential as a renewable resource.
Innovation Solution
A method for producing keratin BD-3 using a nucleic acid molecule encoding the protein, integrated into an expression vector and expressed in host cells, followed by purification and formulation into a pharmaceutical composition for various therapeutic uses.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If keratin is extracted from natural sources, then keratin can be obtained as a renewable resource, but the extraction is very difficult due to insolubility in solvents and resistance to enzymatic hydrolysis
Solution Approach 1:
The patent uses a protein expression system as an intermediary to produce keratin BD-3. Instead of directly extracting from natural sources, the keratin gene is expressed in host cells (E. coli, yeast, or mammalian cells), which then produce the keratin protein that can be purified. This intermediary approach converts an intractable extraction problem into a manageable biotechnological production process.
Solution Approach 2:
The patent changes the production parameter from natural extraction to recombinant expression. By transforming the keratin gene into expression vectors and introducing them into host cells, the production system parameters are fundamentally altered, enabling high-yield production through controlled biological expression rather than difficult chemical extraction.
2Productivity
If keratin is produced through traditional extraction methods, then natural keratin can be obtained, but the production yield is low and the process is complex
Solution Approach 1:
The patent employs host cells that naturally possess the cellular machinery for protein synthesis. These cells self-service by expressing the introduced keratin gene and producing keratin protein autonomously through their own transcription and translation systems, eliminating the need for complex external extraction equipment and processes.
Solution Approach 2:
The patent uses a universal protein expression system that can accommodate different host cells (bacteria, yeast, mammalian cells) and different keratin variants. This multi-functional platform can be adapted to produce various keratin types by simply changing the expression vector or host cell type, rather than requiring specialized extraction procedures for each keratin source.
Data Source
AI summary
The present invention provides a keratin BD-3, a nucleic acid molecule encoding same, an expression vector, a host cell, a preparation method therefor, and a pharmaceutical composition contained the keratin BD-3. The keratin BD-3 can be used in the preparation of medicament for antipyretic, analgesic, antitussive, expectorant, anticonvulsant, antiepileptic, anti-hypertension, anti-inflammatory, and antiviral.