L-Glucose Cultured Tumor Cells for Invasive Cancer Animal Models
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Solution Overview
Problem
Existing cancer model animals, such as those created through subcutaneous or intraperitoneal transplantation, struggle to accurately replicate human invasive cancer and metastasis features, often requiring extracellular matrices like Matrigel and facing challenges in engraftment and reproducibility, which hinders the development of effective cancer detection and treatment technologies.
Innovation Solution
A cancer model animal is created using tumor cells cultured with L-glucose, allowing them to be transplanted subcutaneously or intraperitoneally without Matrigel, resulting in invasive growth and metastasis patterns similar to human cancers, including sarcomas and metastasis to muscles and intra-abdominal organs.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If tumor cells are transplanted subcutaneously or intraperitoneally using conventional methods, then the transplantation procedure is simple, but the tumor cells fail to exhibit invasive growth and metastasis patterns
Solution Approach 1:
The patent changes the chemical composition parameter of the culture medium by replacing D-glucose with L-glucose. This parameter change induces epigenetic modifications in the tumor cells, transforming them from a non-invasive state to an invasive state that exhibits metastasis patterns similar to human cancers, while maintaining the simplicity of subcutaneous or intraperitoneal transplantation procedures
2Reliability
If extracellular matrices like Matrigel are used to support tumor cell transplantation, then tumor cell engraftment is improved, but the model complexity and cost increase
Solution Approach 1:
The patent extracts and removes the requirement for extracellular matrices like Matrigel from the transplantation system. By culturing tumor cells in L-glucose-containing medium, the cells acquire invasive properties that enable them to engraft and metastasize in vivo without any extracellular matrix support, thereby simplifying the model while maintaining reliable engraftment
Solution Approach 2:
The L-glucose-cultured tumor cells become self-sufficient in terms of engraftment support. They inherently possess the ability to invade surrounding tissues and form metastases without requiring external extracellular matrix components, making the system more autonomous and less complex
3Productivity
If traditional anticancer agents are used as first-line treatment, then treatment can be administered immediately, but cancer specificity is low and side effects against normal cells are strong
Solution Approach 1:
The patent performs preliminary action by using L-glucose-cultured tumor cells to create more accurate cancer models and conduct better preclinical trials before human trials. This preliminary modeling phase allows for better prediction of drug efficacy and toxicity, enabling more informed decisions about which agents to advance to clinical testing, ultimately reducing the need for broad-spectrum traditional agents with high side effects
Data Source
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AI summary
Provided are: a cancer model animal in which a malignant tumor corresponding to a pathological image of human invasive cancer or metastasis has been formed or can be formed, despite the use of transplantation under the skin or into the abdominal cavity; and a method for producing the cancer model animal. One aspect of the present invention is: a cancer model animal in which tumor cells that grow in a culture medium containing L-glucose are transplanted under the skin or into the abdominal cavity: a sarcoma model animal in which tumor cells that have been transplanted under the skin or into the abdominal cavity invade muscles; a cancer model animal in which tumor cells, such as adenocarcinoma cells, that have been transplanted under the skin or into the abdominal cavity show metastasis; or a cancer model animal in which tumor cells, such as adenocarcinoma cells, that have been transplanted under the skin or into the abdominal cavity show anomaly of function of orthotopic cells. Another aspect of the present invention is a method for producing a cancer model animal, the method including a step for transplanting a cell composition under the skin or into the abdominal cavity of an animal, the cell composition containing tumor cells that grow by culturing in a culture medium containing L-glucose and being used for producing a cancer model animal by transplanting the cell composition under the skin or into the abdominal cavity of the animal.