Lab-in-Tip Protein Detection for Fast 128-Plex ELISA
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing technologies face challenges in accurately detecting and analyzing proteins and peptides in biological samples, particularly in multiplexed immunoassays, due to issues such as high background signals, reduced signal-to-noise ratios, complex sample preparation, and prolonged reaction times, leading to poor repeatability and false positivity.
Innovation Solution
A protein detection method and device utilizing a Lab-in-Tip technology, incorporating a probe type graphic encoding chip with specific capture antibodies, and sealing SAPE and detection antibodies within a detection tube, enabling quick and efficient multiplexed detection through controlled solvent delivery and reaction with the chip.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If Luminex xMAP technology is used for multiplexed protein detection, then detection throughput and multiplexing capability are improved, but detection background signal increases and signal-to-noise ratio decreases
Solution Approach 1:
The detection system is segmented into distinct functional modules: magnetic bead-based capture units with encoded identifiers, separation magnets for washing and concentration, and detection modules. This segmentation allows each module to perform its function optimally while reducing cross-interference and background signals.
Solution Approach 2:
The invention extracts and removes interfering substances from the sample through magnetic separation and washing steps before detection. The magnetic beads selectively bind target proteins while leaving other serum components in solution, which are then removed during washing, thereby reducing background signal.
2Ease of operation
If traditional ELISA technology is used for protein detection, then detection simplicity is maintained, but repeatability is poor and false positivity occurs
Solution Approach 1:
The magnetic beads perform self-service functions including automatic separation, washing, and concentration of target proteins. The magnetic separation step automatically isolates bead-bound proteins from interfering substances, and the washing steps automatically remove non-specifically bound substances, improving repeatability without requiring complex manual operations.
3Productivity
If suspension array technology is used for multiplexed detection, then detection efficiency is improved, but washing process requires multiple centrifugations and greatly prolongs reaction time
Solution Approach 1:
The invention replaces the mechanical centrifugation system with a magnetic field-based separation system. Magnetic beads respond to magnetic fields for rapid separation and washing, eliminating the need for multiple centrifugation steps and significantly reducing reaction time while maintaining detection efficiency.
4Measurement precision
If Luminex technology optimizes microsphere and detection platforms with enhanced performance, then detection accuracy is improved, but technological complexity and costs increase
Solution Approach 1:
The invention changes key parameters of the detection system: using magnetic beads instead of fluorescent microspheres, magnetic field-based separation instead of flow cytometry, and encoded bead identifiers instead of complex fluorescent signatures. These parameter changes maintain or improve detection accuracy while reducing technological complexity and cost.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves high sensitivity and specificity, completing 128-plexed detection in a single pipette sucker within 1 hour, with reduced sample volume requirements, and provides a simple, cost-effective solution to multiplexed detection, improving detection time and reducing false positivity.
Implementation Method 1
a magnetic bead-based suspension array chip is used for capturing proteins
Implementation Method 2
performing detection and analysis through the probe type graphic encoding chip based on ELISA principle
Data Source
AI summary
A protein detection method and a detection device as well as use are provided. The protein detection method includes: fixing a probe type graphic encoding chip coupled with a specific capture antibody in the detection device, and meanwhile respectively sealing SAPE and a detection antibody in the detection device; and when in detection, performing detection and analysis based on an enzyme linked immunosorbent assay (ELISA) principle through the probe-type graphic encoding chip after the detection antibody and the SAPE are respectively dissolved, so as to obtain protein quantitative analysis results. In the protein detection method, the adopted Lab-in-Tip technology can complete processes such as sampling, washing, detection antibody hybridization, washing and binding of fluorescence labeled streptavidin and a biotin labeled detection antibody in such the Lab-in-Tip device, and has the advantages of multiplexed detection, such as quick completion, convenience, high flexibility, good specificity, few sample capacity and wide detection range.