Lab-on-chip fluid channels for dried blood spot analysis
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Solution Overview
Problem
State-of-the-art dried blood spot (DBS) analysis is limited by the need for off-line manipulations for filter paper-based direct plasma analysis and accurate volume measurements, which are time and cost-consuming, and is not compatible with conventional dried-spot sampling cards like #903® and FTA Elute brand paper.
Innovation Solution
A lab-on-chip device with a card holding element featuring an open cavity and fluid channels that induce capillary action, allowing multiple samplings, fluid filtration, biochemical reactions, and quantitative analysis on conventional sampling cards, including #903® and FTA Elute, with a lid for contact with the outlet to form dried fluid spots.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If filter paper-based DBS analysis is used, then sample collection is simplified and cost-effective, but off-line manipulations are required for direct plasma analysis and volume measurements
Solution Approach 1:
The invention merges the sampling card with integrated fluid channels and reaction zones directly into the filter paper structure, combining sample collection, filtration, biochemical reactions, and analysis functions into a single integrated device, thereby eliminating the need for separate off-line manipulation steps
Solution Approach 2:
The sampling card is designed to perform multiple functions simultaneously: it serves as a collection medium, filtration membrane, reaction chamber, and analysis platform, allowing the same device to handle both simple collection and complex biochemical analysis without requiring additional equipment or steps
2Ease of operation
If conventional dried-spot sampling cards are used, then sample storage and transport are simplified, but they are not compatible with advanced lab-on-chip devices for multiple samplings and biochemical transformations
Solution Approach 1:
The sampling card incorporates multiple independent reaction zones and fluid channels that can perform sequential or parallel biochemical transformations, enabling the same conventional card format to support both simple storage and complex multi-step analytical protocols
Solution Approach 2:
The card is divided into distinct functional zones including collection areas, filtration channels, reaction chambers, and detection regions, allowing each segment to perform its specific function while maintaining overall compatibility with standard sampling card formats
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables direct, efficient, and quantitative analysis of blood or plasma on conventional sampling cards, supporting multiple samplings and biochemical transformations, enhancing selectivity and sensitivity, while reducing the need for off-line manipulations and improving compatibility with various sampling materials.
Implementation Method 1
fluid channels sized in a way as to induce a capillary action
Data Source
AI summary
A lab-on-chip-based system is described for the direct and multiple sampling, control of the volume, fluid filtration, biochemical reactions, sample transfer, and dried spot generation on the conventional and commercial cards for dried fluid spot. Within an all-in-one integrated holder, the invention allows the complete process required to ensure a quantitative analysis of blood, plasma or any other fluids, modification and enrichment of molecule subsets, and formation of a dried fluid spot on the specific spot location of a passive cellulose, non-cellulose, absorbent, or non-absorbent membrane material sampling.