Lactase Formulation With Anions for Sterile Filtration Stability
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Lactase products cause filter clogging during sterilization, leading to increased production costs and delays due to frequent filter replacements.
Innovation Solution
Formulating lactase products with anions such as malate, tartrate, citrate, gluconate, or ethylenediaminetetraacetate to improve physical stability and filterability, reducing pressure buildup in filtration systems.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If lactase product is formulated with only glycerol and tap water, then formulation simplicity is maintained, but filterability deteriorates causing frequent filter clogging
Solution Approach 1:
The patent changes the chemical composition parameters of the lactase formulation by adding specific anions (citrate, tartrate, malate, gluconate, or EDTA) to the existing glycerol and water base. This parameter modification transforms the formulation from causing filter clogging to maintaining filterability, resolving the contradiction between formulation simplicity and filtration efficiency.
Solution Approach 2:
The patent creates a composite formulation by combining lactase with specific anions (citrate, tartrate, malate, gluconate, or EDTA) in addition to the base components. This composite approach enhances the physical stability and filterability of the lactase product, preventing filter clogging while maintaining ease of manufacture.
2Reliability
If lactase product is filtered during sterilization, then sterility is achieved, but filter clogging occurs requiring frequent filter replacement
Solution Approach 1:
The patent applies preliminary action by adding the anion (citrate, tartrate, malate, gluconate, or EDTA) to the lactase formulation before the sterilization filtration step. This pre-treatment modifies the physical properties of the lactase product, preventing aggregation and clogging during the subsequent filtration process, thereby maintaining both sterility and production efficiency.
Solution Approach 2:
The patent changes the physical-chemical parameters of the lactase formulation through anion addition, which modifies the protein stability and prevents aggregation during filtration. This parameter change allows the sterilization filtration to proceed without frequent filter replacements, resolving the contradiction between achieving sterility and avoiding production delays.
3Reliability
If lactase product is filtered during sterilization, then sterility is achieved, but production costs increase due to multiple filter replacements
Solution Approach 1:
The patent applies preliminary action by incorporating the anion (citrate, tartrate, malate, gluconate, or EDTA) into the lactase formulation before sterilization. This pre-treatment prevents filter clogging during the sterilization filtration step, eliminating the need for frequent filter replacements and reducing production costs while maintaining sterility.
Solution Approach 2:
The patent changes the formulation parameters by adding specific anions that improve the physical stability and filterability of lactase. This parameter modification prevents filter clogging during sterilization filtration, reducing the frequency of filter replacements and associated costs while ensuring product sterility.
4Reliability
If lactase product is filtered during sterilization, then sterility is achieved, but filtration pressure builds up reducing filtration capacity
Solution Approach 1:
The patent changes the physical-chemical parameters of the lactase formulation by adding anions (citrate, tartrate, malate, gluconate, or EDTA), which modify the protein conformation and prevent aggregation. This parameter change maintains low filtration pressure during sterilization filtration, allowing efficient processing while achieving sterility.
Solution Approach 2:
The patent creates a composite formulation combining lactase with stabilizing anions that prevent protein aggregation during filtration. This composite approach maintains filtration pressure at acceptable levels while achieving sterility, resolving the contradiction between pressure control and sterilization effectiveness.
Data Source
AI summary
Sterile filtered liquid lactase composition comprising a lactase, preferably a neutral or acidic lactase, an anion selected from malate, tartrate, citrate, gluconate and/or EDTA and a cation selected from sodium and potassium. Preferably, the composition further comprises a polyol. Addition of the said anions to lactase improves filterability by reducing pressure in a filtration system. Preferably the filtration forms part of an in-line filtration system in the production of a dairy product.