Lactase Solution Thermal Stability via Molecular Weight Fraction
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional lactase solutions used for decomposing lactose in milk products suffer from reduced enzyme activity due to thermal instability, particularly when exposed to heat loads above 40°C, leading to incomplete lactose decomposition.
Innovation Solution
A lactase solution with a higher ratio of the 120 kDa lactase fraction, achieved through salting-out and desalting treatments, is developed to enhance thermal stability, maintaining activity even at elevated temperatures.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional lactase solution is used for decomposing lactose in milk, then lactose decomposition can be achieved, but enzyme activity is significantly reduced when exposed to heat loads above 40°C
Solution Approach 1:
The patent applies parameter changes by optimizing the molecular weight distribution of lactase fractions, specifically increasing the proportion of high molecular weight fractions (120 kDa and 80 kDa) while reducing low molecular weight fractions (50 kDa). This compositional parameter change results in enhanced thermal stability, allowing the enzyme to maintain activity at temperatures up to 60°C where conventional lactase would be inactivated.
2Productivity
If lactase is added to raw material milk for lactose decomposition, then lactose can be broken down, but the enzyme becomes inactivated due to heat load at or above 40°C
Solution Approach 1:
The patent changes the molecular weight distribution parameter of the lactase preparation, establishing specific ratio thresholds for different molecular weight fractions. This parameter optimization ensures that the enzyme maintains both high productivity in lactose decomposition and extended duration of action even under thermal stress conditions above 40°C.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The lactase solution effectively decomposes lactose in milk at temperatures up to 60°C with minimal activity reduction, ensuring efficient lactose breakdown in dairy products.
Implementation Method 1
Lactose is a disaccharide composed of galactose and glucose. In order to address lactose intolerance, decomposing in advance the lactose contained in milk or the like to galactose and glucose using the lactase enzyme
Implementation Method 2
a lactase fraction having a molecular weight of about 120 kDa measured by SDS polyacrylamide gel electrophoresis
Data Source
AI summary
[Problem] To provide a lactase solution having excellent thermal stability.[Solution] A lactase solution in which the ratio of a lactase fraction having a molecular weight of about 120 kDa measured by SDS polyacrylamide gel electrophoresis is 20% or more.