Lactate Sensor Using ACES Buffer and Mediator for Rapid Measurement
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Solution Overview
Problem
Conventional lactate sensors have a low enzyme reaction rate, leading to prolonged measurement times and insufficient sensitivity, especially when detecting high lactate concentrations, which is problematic for promptly assessing fatigue levels during training.
Innovation Solution
A lactate sensor incorporating lactate oxidase, a mediator combination of ruthenium compounds and phenazine methosulfate, and N-(2-acetamide)-2-aminoethanesulfonic acid (ACES) as a buffer, enhancing enzyme activity and reaction rates to enable faster and more accurate lactate concentration measurements.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional lactate sensors use standard enzyme reaction systems, then the sensor structure is simple, but the measurement time is prolonged and sensitivity is insufficient
Solution Approach 1:
The patent changes the chemical parameters of the reaction system by introducing ACES buffer and a specific mediator combination (ruthenium compound and phenazine methosulfate), which fundamentally alters the reaction kinetics and enables both high sensitivity and rapid measurement
Solution Approach 2:
The patent uses a mediator system consisting of ruthenium compound and phenazine methosulfate that facilitates electron transfer between lactate oxidase and the electrode, significantly enhancing the reaction rate and sensitivity while reducing measurement time
2Speed
If conventional lactate sensors are used, then the device complexity is low, but the enzyme reaction rate is insufficient for rapid measurement
Solution Approach 1:
The patent employs a composite reagent system combining multiple components (lactate oxidase, ACES buffer, ruthenium compound, and phenazine methosulfate) that work synergistically to achieve high reaction rates, accepting the necessary complexity in exchange for performance
3Measurement precision
If conventional sensors measure high lactate concentrations, then the measurement range is adequate, but the sensitivity is insufficient
Solution Approach 1:
The patent optimizes the pH buffer system using ACES, which maintains optimal enzyme activity across a broader concentration range, enabling high sensitivity measurements even at elevated lactate concentrations
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The lactate sensor achieves rapid and accurate lactate concentration measurements, even at high concentrations, significantly reducing measurement time and improving sensitivity, making it suitable for real-time fatigue assessment during training.
Implementation Method 1
a reagent layer containing lactate oxidase, a mediator, and N-(2-acetamide)-2-aminoethanesulfonic acid (ACES)
Implementation Method 2
electrochemically measuring a reaction between lactate in the sample and the lactate oxidase via a mediator
Implementation Method 3
the mediator is a combination of a ruthenium compound, and phenazine methosulfate (PMS) or a derivative thereof
Data Source
AI summary
A lactate sensor capable of accurately measuring a lactate concentration in a short period of time. The lactate sensor includes an insulating substrate, an electrode system including at least a working electrode and a counter electrode provided on the substrate, and a reagent layer provided on the electrode system. The reagent layer contains lactate oxidase, a mediator, and N-(2-acetamide)-2-aminoethanesulfonic acid.