Lactic Acid Bacterial Strains for High-Purity Nanoparticle Synthesis
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for synthesizing zinc oxide, silver, and silver chloride nanoparticles from lactic acid bacteria are limited in terms of purity and efficiency, and there is a need for new bacterial strains that can effectively produce these nanoparticles for antimicrobial applications.
Innovation Solution
The development of new lactic acid bacterial strains of Latilactobacillus curvatus, Limosilactobacillus fermentum, and Lactiplantibacillus plantarum, deposited in the Polish Collection of Microorganisms, which are used to isolate and synthesize zinc oxide, silver, and silver chloride nanoparticles, including hybrid systems, using specific cultural and centrifugation methods to enhance yield and purity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If chemical methods are used to synthesize nanoparticles, then production efficiency is high, but product purity and antimicrobial effectiveness are limited
Solution Approach 1:
The patent introduces lactic acid bacteria as a biological intermediary mediator between metal precursors and nanoparticle formation. The bacteria produce extracellular polymeric substances and metabolites that act as reducing and stabilizing agents, enabling controlled nanoparticle synthesis with high purity while maintaining efficient production rates. This biological mediation resolves the contradiction by providing a natural catalyst that enhances both purity and effectiveness.
Solution Approach 2:
The patent optimizes multiple parameters including bacterial culture conditions (pH, temperature, incubation time), metal precursor concentration, and nutrient composition to maximize nanoparticle yield and purity. By systematically adjusting these parameters, the method achieves high production efficiency while maintaining superior product quality and antimicrobial effectiveness compared to conventional chemical methods.
2Ease of manufacture
If conventional lactic acid bacteria strains are used, then isolation and cultivation are straightforward, but nanoparticle synthesis efficiency and purity are limited
Solution Approach 1:
The patent performs preliminary selection and optimization of lactic acid bacteria strains before nanoparticle synthesis. Specific strains are pre-screened for their metabolic profiles and nanoparticle production capabilities, and culture conditions are pre-optimized to maximize extracellular polymer production. This preliminary preparation ensures that subsequent nanoparticle synthesis proceeds with high efficiency and purity while maintaining ease of cultivation.
3Productivity
If high concentrations of metal precursors are used to increase nanoparticle yield, then productivity improves, but product purity decreases due to residual ions and contaminants
Solution Approach 1:
The bacterial extracellular polymeric substances act as a protective intermediary that stabilizes metal ions during reduction and prevents aggregation. This natural capping agent allows high precursor concentrations to be used for maximum yield while the bacterial matrix prevents contamination and facilitates easy separation, maintaining high product purity even at elevated production levels.
Solution Approach 2:
The patent replaces complex chemical purification mechanisms with biological separation. The bacterial cells and their extracellular products naturally adsorb and stabilize nanoparticle surfaces, allowing simple centrifugation and filtration to achieve high purity products. This substitution of chemical purification with biological stabilization resolves the yield-purity contradiction.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The new bacterial strains enable the efficient synthesis of high-purity zinc oxide, silver, and silver chloride nanoparticles, demonstrating enhanced antimicrobial activity against a range of bacteria and fungi, with improved stability and effectiveness compared to chemically derived nanoparticles.
Implementation Method 1
A method for obtaining antibacterial zinc oxide nanocomposites by extracellular biosynthesis using a supernatant obtained from the culture of a strain of lactic acid bacteria and a precursor in the form of zinc nitrate
Implementation Method 2
The culture is then centrifuged at 10000-12000 rpm (preferably 12000 rpm) for 5-20 min. (preferably 15 min), filtered
Implementation Method 3
silver nitrate in the form of crystals is added in the amount from 0.129gIL to 0. 645 g/L with a final concentration from 1 to 5 mM
Implementation Method 4
it is incubated in the light-free medium at 27-40 °C (preferably 36 °C) for 24-36 h (preferably 30 h)
Implementation Method 5
where the medium has a composition of ammonium citrate at a concentration of 0.5-5 g/L, di-Potassium hydrogen phosphate at a concentration of 0.5 to 5 g/L, glucose at a concentration from 15 to 30 g/L
Data Source
Figure 1~2
Figure 3~4
Figure 5~6
AI summary
The objects of the inventions are new lactic acid bacterial strains Latilactobacillus curvatus, Limosilactobacillus fermentum and Lactiplantibacillus plantarum for antimicrobial applications, a method for isolating the lactic acid bacterial strains Latilactobacillus curvatus, Limosilactobacillus fermentum and Lactiplantibacillus plantarum and a method for obtaining silver nanoparticles, silver chloride nanoparticles and hybrids of silver nanoparticles and silver chloride and zinc oxide nanoparticles from the lactic acid bacterial strain Latilactobacillus curvatus, Limosilactobacillus fermentum and Lactiplantibacillus plantarum.