Lactic Acid Bacteria Probiotic Composition for Infection Treatment
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Solution Overview
Problem
Current treatments for urogenital and gastrointestinal infections often rely on antibiotics, which can disrupt the natural flora and lead to side effects, while existing probiotic strains may not be sufficiently effective in preventing pathogen overgrowth and colonization.
Innovation Solution
The use of specifically selected probiotic strains from the Lactobacillus genus, such as L. acidophilus, L. crispatus, L. gasseri, L. helveticus, and L. jensenii, which adhere to epithelial cells, resist gastric environment, inhibit pathogen adhesion and internalization, and modulate the immune response, administered with a suitable delivery system to restore and maintain healthy flora.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If antibiotics are used to treat urogenital and gastrointestinal infections, then pathogen elimination is achieved, but natural flora is disrupted and side effects occur
Solution Approach 1:
The patent converts the harmful effect of antibiotics on natural flora into a benefit by using probiotic bacteria that restore and maintain healthy flora. The probiotics selectively colonize the gut and urogenital tracts, producing beneficial substances that inhibit pathogens without disrupting existing beneficial bacteria, thus transforming the harmful antibiotic-flora interaction into a beneficial probiotic-flora restoration process
Solution Approach 2:
The patent introduces probiotic bacteria as intermediary agents between the host and pathogens. These probiotics act as mediators that compete with pathogens for adhesion sites and resources, produce antimicrobial substances, and modulate immune responses, thereby eliminating the need for direct antibiotic intervention and its associated harmful effects
2Object-generated harmful factors
If conventional probiotic strains are used, then natural alternative to antibiotics is provided, but sufficient effectiveness in preventing pathogen overgrowth is not achieved
Solution Approach 1:
The patent applies local quality by selecting and optimizing specific probiotic strains (Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus helveticus, Lactobacillus jensenii) that are particularly effective in specific locations (gut and urogenital tracts). Each strain is chosen for its localized colonization capability and specific antimicrobial properties against relevant pathogens in that compartment
Solution Approach 2:
The patent employs parameter changes by modifying probiotic strain characteristics through selective pressure and optimization. The strains are selected for specific properties such as adhesion to epithelial cells, resistance to gastric environment, production of hydrogen peroxide and lactic acid, and ability to inhibit pathogen internalization, thereby enhancing their effectiveness beyond conventional probiotics
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
These probiotic strains effectively prevent and treat urogenital and gastrointestinal infections by establishing a balanced flora, inhibiting pathogen growth, and modulating the immune response, offering a natural alternative to antibiotics with improved efficacy.
Implementation Method 1
The probiotic bacteria strains used within the frame of this invention have been first selected for their ability to adhere to epithelial cells... These strains further exhibit a significant resistance... and a fairly good to excellent resistance when exposed to enzymes like pepsin and pancreatin... The said probiotics have been further selected for their additional ability to inhibit adhesion, growth and even survival of pathogens
Implementation Method 2
The most efficient LAB within that frame are those which express high amounts of both hydrogen peroxide (H2O2) and lactic acid in situ where both factors act synergistically
Implementation Method 3
The probiotic bacteria strains used within the frame of this invention have been first selected for their ability to adhere to epithelial cells such cervix HeLa or Caco-2
Implementation Method 4
These strains further exhibit a significant resistance, i.e. survival rate when exposed to the highly acidic gastric environment and a fairly good to excellent resistance when exposed to enzymes like pepsin and pancreatin
Data Source
AI summary
Useful probiotics have been selected among lactic acid bacteria strains of the genus L. acidophilus, L. crispatus, L. gasseri, L. helveticus and L. jensenii for their ability to kill urogenital and/or gastrointestinal pathogens and their ability to inhibit internalization of urogenital and/or gastrointestinal pathogens within urogenital and/or gastrointestinal epithelial cells in humans. Probiotic compositions comprise at least one of the said lactic acid bacteria strains in combination with a suitable delivery system, such as a food product or a beverage, a food or beverage compositions, a food or beverage supplement or adjuvant.