Lactic Acid Bacteria Activation via Citrate Electron Acceptors
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Solution Overview
Problem
The oral administration of live bacteria often results in insufficient activity in the intestinal tract, leading to increased dosages and frequency of administration, which can be costly and ineffective, particularly in neonates where nutrient restrictions are necessary for medical reasons.
Innovation Solution
The use of citrate as an external electron acceptor in combination with a carbon source like lactose enhances the reconstitution and growth of specific live bacteria strains, such as Lactobacillus reuteri DSM 17938, to achieve faster activation with minimal impact on nutrient content.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If the dosage of live bacteria is increased to ensure sufficient activity in the intestinal tract, then the effectiveness of the therapy is improved, but the cost increases and the frequency of administration becomes more burdensome
Solution Approach 1:
The patent applies preliminary action by providing a pre-formulated composition containing specific nutrients (proteins, lipids, carbohydrates, vitamins, minerals) that are prepared in advance to optimize bacterial activation and growth. This pre-prepared nutritional support ensures that when bacteria are administered, they immediately have the necessary components for rapid activation and proliferation in the gastrointestinal tract, eliminating the need for higher dosages or more frequent administration
Solution Approach 2:
The patent uses an intermediary approach by introducing a specific nutritional composition that acts as a mediator between the administered bacteria and the host's gastrointestinal environment. This composition includes proteins, lipids, carbohydrates, vitamins, and minerals that facilitate optimal bacterial growth and activation, allowing lower bacterial dosages to achieve the same therapeutic effect
2Reliability
If the frequency of administration is increased to maintain sufficient bacterial activity, then the therapeutic effectiveness is improved, but the burden on the patient increases and cost rises
Solution Approach 1:
The pre-formulated nutritional composition is designed to sustain bacterial activity over extended periods, allowing for less frequent administration. The comprehensive nutrient profile ensures that bacteria remain active and proliferate effectively throughout the dosing interval, reducing the need for frequent re-administration
Solution Approach 2:
The patent ensures continuity of useful action by providing a nutritional composition that maintains bacterial metabolism and growth continuously over time. The balanced formulation of proteins, lipids, carbohydrates, vitamins, and minerals ensures sustained bacterial activity, allowing for extended intervals between administrations while maintaining therapeutic effectiveness
3Object-affected harmful factors
If nutrients are restricted for medical reasons in neonates, then the health condition is managed, but the activation and growth of administered bacteria is hindered
Solution Approach 1:
The patent applies local quality by providing a targeted nutritional composition specifically formulated to support bacterial growth in the gastrointestinal tract without affecting the overall nutrient restriction regimen. The composition includes proteins, lipids, carbohydrates, vitamins, and minerals in specific proportions that locally enhance bacterial activation and proliferation while maintaining the necessary nutrient restrictions for the neonate's medical condition
Solution Approach 2:
The nutritional composition acts as an intermediary that bridges the conflict between nutrient restriction and bacterial growth requirements. It provides the necessary nutrients for bacterial activation in a controlled, localized manner that does not compromise the overall nutrient management strategy for the neonate's health condition
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for quicker and more effective activation of live bacteria, potentially reducing the required dosage and frequency of administration, while maintaining minimal nutritional influence, thereby enhancing therapeutic benefits in neonates.
Implementation Method 1
L. reuteri is a heterofermentative lactic acid bacterium... Strains that can use citrate as an electron acceptor in their metabolism
Implementation Method 2
a carbon source, such as lactose... improved the reconstitution and early growth of L. reuteri DSM 17938
Data Source
AI summary
The present invention relates to methods of activating live lactic acid bacteria comprising exposing said bacteria to a preparation comprising citrate, wherein said bacteria have the ability to utilize citrate as an external electron acceptor. The present invention further relates to methods to enhance the activity of certain live bacteria in mammals. More specifically the invention relates to improve the wake-up of certain lactic acid bacteria from the freeze-dried state. The present invention also relates to preparations comprising said activated bacteria and therapeutic uses of said activated bacteria.