Lactobacillus plantarum K8 Aglycone Bioconversion
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for converting glycosides into aglycones are inefficient, leading to low production rates, high production costs, and potential contamination during purification, particularly in the bioconversion of isoflavones and saponins using lactic acid bacteria.
Innovation Solution
A method involving a microorganism, specifically Lactobacillus plantarum K8, that produces β-glycosidase to convert glycosides into aglycones, which are then accumulated in the microorganism's cells, allowing for a high concentration of aglycones to be used as a raw material.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional extraction methods (mechanical/chemical) are used to extract aglycone from plants, then extraction can be achieved, but production rate decreases and production cost increases
Solution Approach 1:
The patent replaces mechanical extraction methods (ultrasonic waves, liquid chromatography) with a biological conversion system using lactic acid bacteria that produce β-glycosidase. The bacteria convert glycoside directly into aglycone through enzymatic action, eliminating the need for complex mechanical and chemical extraction processes, thereby increasing production rate while maintaining aglycone concentration.
Solution Approach 2:
The patent changes the fundamental parameter of the conversion process from mechanical/chemical extraction to biological enzymatic conversion. By using lactic acid bacteria with β-glycosidase activity, the process transforms glycoside to aglycone in situ, fundamentally altering the production mechanism to achieve both high concentration and high productivity.
2Quantity of substance
If conventional extraction methods are used, then aglycone can be obtained, but purification process increases contamination risk
Solution Approach 1:
The patent replaces mechanical extraction and purification systems with a biological system where lactic acid bacteria perform both conversion and natural purification. The bacterial cells themselves become the purification medium, converting glycoside to aglycone and naturally separating it from contaminants, thereby eliminating contamination risks associated with chemical solvents and complex purification equipment.
3Ease of operation
If glycoside is used directly without bioconversion, then simple administration is possible, but absorption rate is low
Solution Approach 1:
The patent performs preliminary bioconversion of glycoside to aglycone using lactic acid bacteria before administration. The bacteria are administered along with the glycoside, and the β-glycosidase they produce converts the glycoside to aglycone in the gastrointestinal tract, creating the highly absorbable form in advance of absorption, thereby maintaining administration simplicity while dramatically increasing absorption rate.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly increases the concentration of aglycones, improving bioavailability and absorption rates, as demonstrated by the 1,300 times higher absorption rate of aglycone saponin compared to non-modified ginsenoside in animal tests, and enables their selective uptake and release into the bloodstream.
Implementation Method 1
converting a glycoside into an aglycone form by using a microorganism producing β-glycosidase
Implementation Method 2
β-glycosidase in the intestines to be converted into an aglycone as an active aglycone and absorbed
Implementation Method 3
having the aglycone accumulated in the cells
Data Source
Figure 1
Figure 2
Figure 3
AI summary
The present invention relates to a method for preparing an aglycone converted from a glycoside and, specifically, to a method for preparing an aglycone from a glycoside by converting a glycoside into an aglycone form by using a microorganism producing β-glycosidase, and then recovering the aglycone accumulated in the cells of the microorganism.