Lactobacillus Acidophilus Strain Selection for BSH-Driven Cholesterol Lowering
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Solution Overview
Problem
The increasing incidence of metabolic syndrome due to obesity, characterized by hypertension, insulin resistance, type 2 diabetes, dyslipidemia, and non-alcoholic fatty liver disease, necessitates the development of effective interventions to reduce serum cholesterol levels and body fat percentage to prevent cardiovascular diseases.
Innovation Solution
Isolation and screening of Lactobacillus acidophilus GOLDGUT-LA100 from the human intestinal tract, identified by bacterial morphology and 16S rDNA sequencing, which exhibits high bile salt hydrolase activity, gastric acid tolerance, and effective in reducing weight, blood glucose, and serum cholesterol levels in animal models.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional lactic acid bacteria are used, then general probiotic effects are achieved, but bile salt hydrolase activity is insufficient for effective cholesterol lowering
Solution Approach 1:
The patent changes the key parameter of BSH activity by selecting and screening specific Lactobacillus acidophilus strains (LA-01, LA-02, LA-03, LA-04, LA-05) that exhibit high BSH activity. This parameter change transforms ordinary probiotics into specialized cholesterol-lowering agents, directly resolving the contradiction between general probiotic effects and sufficient BSH activity for effective cholesterol reduction.
2Reliability
If high doses of probiotics are administered to achieve therapeutic effects, then cholesterol lowering and weight loss are improved, but gastric acid tolerance becomes a limiting factor
Solution Approach 1:
The patent performs preliminary selection and screening of Lactobacillus acidophilus strains with inherently high BSH activity and gastric acid tolerance before administration. By pre-identifying strains that can withstand gastric acid conditions and maintain high BSH activity, the patent ensures therapeutic effectiveness without requiring excessive dosages, thus resolving the contradiction between therapeutic effect and gastric acid tolerance.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Lactobacillus acidophilus GOLDGUT-LA100 demonstrates significant weight loss, blood glucose lowering, and serum cholesterol reduction, along with liver fat deposition and adipose tissue inflammation alleviation, providing a basis for probiotic products with lipid-lowering and weight management benefits.
Implementation Method 1
The bile salt hydrolase (BSH) produced by intestinal bacteria can hydrolyze combined glycine bile salts and taurine bile salts into free bile salts and amino acid residues
Implementation Method 2
The synthesis of the bile salts in the liver requires the migration of cholesterol from the blood to the liver, thereby reducing the serum total cholesterol level
Implementation Method 3
the lipid emulsifying capacity of the small intestine is limited
Data Source
AI summary
The present invention relates to the technical field of microorganisms, in particular to a Lactobacillus acidophilus GOLDGUT-LA100 having functions of lipid lowering, blood glucose lowering and weight loss and application thereof. The Lactobacillus acidophilus GOLDGUT-LA100 provided by the present invention is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 28398. The strain has strong acid resistance, can adapt to the digestive tract environment, and has high bile salt hydrolase activity. At the same time, in animal experiments, the strain significantly reduces blood glucose and serum cholesterol levels, reduces liver fat deposition, reduces adipose tissue inflammation, effectively reduces the weight, and has broad application prospects in the development of products for improving obesity, lowering lipid and lowering blood glucose.