Lactobacillus plantarum Inducia Strain for LDL Reduction
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Solution Overview
Problem
Current probiotics are ineffective in consistently reducing LDL-cholesterol levels and oxidative stress markers, such as ox-LDL, and do not adequately prevent cholesterol metabolism disorders and cardiovascular diseases, with some strains failing to demonstrate significant anti-Clostridium difficile activity.
Innovation Solution
The use of Lactobacillus plantarum Inducia DSM 21379 strain, which exhibits high bile salt hydrolase activity and antimicrobial properties, is proposed for reducing LDL-cholesterol levels, oxidative stress markers, and inhibiting Clostridium difficile growth, formulated into food products, supplements, or pharmaceuticals.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current probiotics are used, then general probiotic effects are provided, but LDL-cholesterol levels and oxidative stress markers are not consistently reduced
Solution Approach 1:
The patent selects a specific strain Lactobacillus plantarum Inducia DSM 21379 with optimized parameters including high BSH activity (parameter) and specific antimicrobial properties. This strain-specific approach changes the parameter of probiotic efficacy to achieve consistent LDL-cholesterol reduction and ox-LDL reduction that generic probiotics fail to deliver.
2Reliability
If probiotics with BSH activity are used, then cholesterol lowering ability is improved, but antimicrobial activity against pathogenic strains varies among strains
Solution Approach 1:
The patent identifies a probiotic strain Lactobacillus plantarum Inducia DSM 21379 that simultaneously performs multiple functions: high BSH activity for cholesterol lowering, antimicrobial activity against Clostridium difficile and other pathogenic strains, and reduction of oxidative stress markers. This multi-functional strain resolves the contradiction by providing both reliable cholesterol management and broad antimicrobial protection.
3Object-affected harmful factors
If broad-spectrum antimicrobial preparations are used, then infections are treated, but microbial balance in gastrointestinal tract is disrupted
Solution Approach 1:
The patent uses Lactobacillus plantarum Inducia DSM 21379 as a intermediary organism that restores microbial balance after antibiotic treatment. The strain produces antimicrobial substances that target pathogenic bacteria while being compatible with beneficial gut microbiota, thus treating infections without disrupting the overall microbial ecosystem.
4Reliability
If probiotics are used for prevention, then health benefits are provided, but significant anti-Clostridium difficile activity is not achieved with some strains
Solution Approach 1:
The patent selects a specific strain Lactobacillus plantarum Inducia DSM 21379 with optimized parameters including high BSH activity and specific antimicrobial properties. This strain-specific approach changes the parameter of probiotic efficacy to achieve consistent LDL-cholesterol reduction and ox-LDL reduction that generic probiotics fail to deliver.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Lactobacillus plantarum Inducia DSM 21379 effectively decreases LDL-cholesterol levels, reduces oxidative stress markers, and demonstrates significant antimicrobial activity against Clostridium difficile, providing cardio-protective and anti-inflammatory benefits.
Implementation Method 1
BSH (EC 3.5.1.24; cholylglycine hydrolase) is an enzyme that catalyses the hydrolysis of glycine- and/or taurine-conjugated bile salts into amino acid residues and free bile acids
Implementation Method 2
lactic acid bacteria assimilate cholesterol from dietary products
Implementation Method 3
the ability of LDL-c to independently cause development and progression of coronary heart disease... Oxidized low-density lipoprotein (Ox-LDL) is a marker of oxidative stress specific to LDL particles
Implementation Method 4
demonstrated the cholesterol lowering ability. These strains have bile salt hydrolases (BHS) activity, also the antagonistic activity to inhibit the growth of pathogenic strains
Data Source
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AI summary
The invention relates to a microorganism strain Lactobacillus plantarum Inducia DSM 21379 for use as hypocholesterolemic, antimicrobial and anti-oxidative agent and as enhancer of the natural defense potential of a subject. L. plantarum Inducia is used for reducing the level of LDL-cholesterol in blood for preventing the cholesterol metabolism disorders and cardiovascular disorders. L. plantarum Inducia enhances simultaneously the natural defense potential and cellular immunity, and as anti-oxidative agent enhances anti-oxidative activity of human body by reducing the level of oxidized low density lipoprotein (ox-LDL). The strain is also used as an antimicrobial agent for lowering risk of Clostridium difficile associated diarrhoeae (CDAD) by preventing germination of C. difficile spores and by suppressing proliferation of its vegetative cells. The compositions comprising L. plantarum Inducia decrease the levels of LDL-cholesterol and ox-LDL in blood, enhance the natural defense potential and lower risk of CDAD. The composition may further comprise xylitol.