Lactobacillus Strains Inhibit Oral Pathogens
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Solution Overview
Problem
Current methods for preventing oral pathogens and promoting oral health in children are inadequate, as evidenced by high caries rates in Taiwanese children, despite existing fluoridizing policies, and only a few Lactobacillus strains have been proven effective in inhibiting oral pathogens and whitening teeth.
Innovation Solution
A method involving food, oral cleaning, and medical composites containing specific Lactobacillus strains such as Lactobacillus acidophilus F-1, Lactobacillus salivarius subsp. salicinius AP-32, Lactobacillus reuteri GL-104, Lactobacillus paracasei GL-156, Lactobacillus helveticus RE-78, Lactobacillus rhamnosus CT-53, and Lactobacillus paracasei ET-66, which are isolated and preserved in reputable culture collections, to inhibit oral pathogens and promote oral health by competing with pathogens for space and nutrition, and secreting hydrogen peroxide for odor elimination and teeth whitening.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If fluoridizing policy is implemented, then caries prevalence rate decreases, but caries protection rate remains low compared to WHO targets
Solution Approach 1:
The patent changes the approach from chemical fluoridization to biological probiotic intervention. Specific Lactobacillus strains (L. acidophilus F-1, L. salivarius AP-32, L. reuteri GL-104, L. paracasei GL-156, L. helveticus RE-78, L. rhamnosus CT-53, L. paracasei ET-66) are selected and applied to modify the oral microecological system, providing superior caries protection that meets WHO targets
Solution Approach 2:
The patent introduces probiotic Lactobacillus strains as intermediary organisms that compete with cariogenic bacteria for adhesion sites and nutrients. These probiotics produce bacteriocins and hydrogen peroxide that inhibit pathogenic bacteria, acting as biological mediators to achieve effective caries prevention
2Reliability
If conventional oral hygiene methods are used, then basic cleaning is achieved, but oral pathogen inhibition is insufficient
Solution Approach 1:
The patent introduces probiotic Lactobacillus strains as intermediary organisms that compete with cariogenic bacteria for adhesion sites and nutrients. These probiotics produce bacteriocins and hydrogen peroxide that inhibit pathogenic bacteria, acting as biological mediators to achieve effective caries prevention
Solution Approach 2:
The probiotic Lactobacillus strains establish themselves in the oral cavity and continuously provide protective functions through self-replication and metabolite production. The bacteria autonomously compete with pathogens for resources and produce inhibitory substances, providing ongoing protection without requiring complex external intervention systems
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The described Lactobacillus strains effectively inhibit Streptococcus mutans, Fusobacterium nucleatum subsp. polymorphum, Aggregatibacter actinomycetemcomitans, and Porphyromonas gingivalis, reducing oral pathogen loads and promoting oral health, with strains like Lactobacillus rhamnosus CT-53 showing significant caries prevention and teeth whitening capabilities.
Implementation Method 1
probiotics compete with pathogens for the living area they attach to and the nutrition they feed on
Implementation Method 2
The probiotics also secrete bacteria-inhibiting material, which can weaken pathogens or even kill pathogens
Implementation Method 3
the probiotics also secrete hydrogen peroxide, which can eliminate oral odor and whiten teeth
Data Source
AI summary
A method of inhibiting oral pathogens comprising administering to a subject in need thereof an effective amount of Lactobacillus strain-containing food composite, medical composite and oral cleaning composite. The food composite, medical composite and oral cleaning composite comprises at least one Lactobacillus strain, which is at least one isolated Lactobacillus strain selected from a group including Lactobacillus acidophilus F-1 strain, CCTCC NO: M2011124; Lactobacillus salivarius subsp. salicinius AP-32 strain, CCTCC NO: M2011127; Lactobacillus reuteri GL-104 strain, CCTCC NO: M209138; Lactobacillus paracasei GL-156 strain, CCTCC NO: M2014590; Lactobacillus helveticus RE-78 strain, CGMCC No. 13513; Lactobacillus rhamnosus CT-53 strain, CCTCC NO: M2011129; and Lactobacillus paracasei ET-66 strain, CGMCC No. 13514, which are respectively preserved in China Center for Type Culture Collection (CCTCC) and China General Microbiological Culture Collection Center (CGMCC).