Lactobacillus paracasei 207-27 Gastric Acid and Bile Salt Tolerance

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Solution Overview

Problem

Current probiotics face challenges in surviving the acidic environment of the gastric juice and bile salts, which hinders their ability to effectively colonize the intestine and provide immune regulation and anti-allergic benefits.

Innovation Solution

The development of Lactobacillus paracasei 207-27, which has been identified for its high tolerance to gastric acid and bile salts, and its ability to adhere to the intestinal tract, enhancing immunity and alleviating allergic symptoms, is used in compositions, food products, and dietary supplements to promote immune function and reduce inflammation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional probiotics are administered, then immune regulation and anti-allergic benefits are expected, but the probiotics cannot survive the acidic environment of gastric juice and bile salts, hindering their ability to colonize the intestine

Engineering Contradiction:

Improvesurvival rate of probioticsVSAvoidacidic environment tolerance

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent applies parameter changes by selecting and characterizing Lactobacillus paracasei 207-27 based on its specific physiological parameters - particularly its tolerance to acidic environments (pH 2.0 for 3 hours) and bile salts (0.3% w/v for 3 hours). This strain demonstrates survival rates of over 80% under these harsh conditions, which is a key parameter that distinguishes it from conventional probiotics and enables effective intestinal colonization.

Inventive Principle:

Principle #35Parameter changes

2Reliability

If probiotics are to effectively colonize the intestine and provide health benefits, then they must tolerate gastric acid and bile salts, but most probiotic strains lack this tolerance and die before reaching the intestine

Engineering Contradiction:

Improvecolonization abilityVSAvoidgastric and bile salt resistance

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent employs self-service by utilizing the inherent properties of Lactobacillus paracasei 207-27 itself to solve the survival problem. The strain naturally produces acid-resistant and bile-resistant characteristics without requiring external protection mechanisms. It autonomously maintains viability through its cellular adaptations to harsh gastrointestinal conditions, enabling it to reach and colonize the intestine independently.

Inventive Principle:

Principle #25Self-service

3Reliability

If probiotics need to adhere to intestinal epithelial cells and colonize the intestine to produce beneficial metabolites, then they must survive the upper gastrointestinal tract, but conventional probiotics are eliminated by the defense mechanism before they can establish colonization

Engineering Contradiction:

Improveadhesion to intestinal epitheliumVSAvoidgastrointestinal defense mechanism

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent applies preliminary action by pre-screening and selecting Lactobacillus paracasei 207-27 for its demonstrated ability to withstand gastric acid and bile salt exposure before intestinal colonization. The strain has been verified to maintain viability through the upper gastrointestinal tract barriers, ensuring it reaches the intestine in sufficient numbers to establish adhesion and colonization on intestinal epithelial cells.

Inventive Principle:

Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Lactobacillus paracasei 207-27 effectively survives gastric and bile environments, adheres to the intestinal mucosa, activates macrophages, increases anti-inflammatory cytokine expression, and reduces serum IgE levels, thereby improving immune function and alleviating allergic reactions.

Implementation Method 1

The Lactobacillus paracasei of the present application has good tolerance to the acidic environment of gastric juice and bile salts

Methodology Applied

Scientific EffectAcid tolerance:

Implementation Method 2

The Lactobacillus paracasei of the present application has good tolerance to the acidic environment of gastric juice and bile salts

Methodology Applied

Scientific EffectBile salt tolerance:

Implementation Method 3

has high capability to adhesion to the intestinal tract

Methodology Applied

Scientific EffectAdhesion: Adhesive

Implementation Method 4

can activate the activity of macrophages and promote the expression of cytokines

Methodology Applied

Scientific EffectImmune activation:

Implementation Method 5

significantly increase the expression of anti-inflammatory factor IL-10

Methodology Applied

Scientific EffectCytokine expression:

Implementation Method 6

can reduce the level of serum IgE and improve allergic reactions

Methodology Applied

Scientific EffectIgE reduction:

Data Source

PatentUS11812770B2<i>Lactobacillus paracasei </i>207-27 and use thereof

Publication Date: 2023.11.14 BY HEALTH CO LTD

AI summary

The present application relates to a Lactobacillus paracasei or progeny thereof. Specifically, the present application relates to Lactobacillus paracasei 207-27 and a composition, culture, food product or dietary supplement containing the same. The present application also relates to the use of Lactobacillus paracasei 207-27 and the composition, culture, food product or dietary supplement containing the same in medicine.