Lactobacillus paracasei LMG S-26420 for High-Yield CLA Production
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Solution Overview
Problem
Current bacterial strains used for converting linoleic acid (LA) into conjugated linoleic acid (CLA) have low productivity yields, making it difficult to obtain sufficient CLA for health benefits, especially in industrial scale processes, and there is a need for a strain that can efficiently produce the biologically active isomers c9-t11 and t10-c12.
Innovation Solution
The development of Lactobacillus paracasei LMG S-26420, which converts LA into CLA with a higher percentage than other known lactobacilli, producing prevalent isomers c9-t11 and t10-c12, and can be obtained in high productivity yields, stable in lyophilic form for long-term storage and use in nutritional or pharmaceutical compositions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional bacterial strains are used for converting linoleic acid into conjugated linoleic acid, then the conversion process can proceed, but the productivity yield is low making it difficult to obtain sufficient CLA for health benefits
Solution Approach 1:
The patent applies parameter changes by optimizing culture conditions including pH (maintained between 6.0-7.5 using ammonium acetate buffer), temperature (37°C incubation), and nutrient composition (MRS medium with specific additives like cysteine hydrochloride and Tween 80) to maximize CLA production yield and achieve high productivity
2Productivity
If high productivity yields are required for industrial scale processes, then sufficient CLA can be produced, but current strains cannot achieve the necessary conversion efficiency
Solution Approach 1:
The patent applies preliminary action by pre-adapting the Lactobacillus paracasei strain to linoleic acid through pre-cultivation steps, and by pre-optimizing all culture parameters before the main production phase, ensuring the bacteria are ready to convert LA to CLA at maximum efficiency from the start of industrial scale production
3Duration of action of stationary object
If the strain can be stored long-term, then stability is improved, but cell viability may be compromised during storage
Solution Approach 1:
The patent applies phase transitions by using lyophilization (freeze-drying) to convert the bacterial culture from liquid to solid dry powder form, removing water to prevent metabolic activity and degradation, thereby enabling long-term storage at room temperature while preserving cell viability and CLA production capability
Solution Approach 2:
The patent uses protective agents such as skim milk powder and other excipients as intermediaries during lyophilization to protect bacterial cells from damage during the drying process and storage, maintaining cell viability throughout long-term storage
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Lactobacillus paracasei LMG S-26420 achieves a CLA conversion percentage higher than 30%, with isomers c9-t11 and t10-c12 being prevalent, and maintains high cell viability during lyophilization, enabling effective production and storage for use in treating CLA deficiency-related disorders and maintaining intestinal flora balance.
Implementation Method 1
Lactobacillus paracasei LMG S-26420, able to convert linoleic acid (LA) into conjugated linoleic acid (CLA)
Implementation Method 2
maintains high cell viability during lyophilization, enabling effective production and storage
Data Source
AI summary
The present invention refers to a new strain belonging to the species Lactobacillus paracasei able to convert linoleic acid into conjugated linoleic acid.The present invention refers also to nutritional or food preparations and/or pharmaceutical compositions, comprising the strain Lactobacillus paracasei, useful in the treatment and/or prevention of pathologies and/or physiological states related to conjugated linoleic acid deficiency or in the cases wherein the use of a probiotic is beneficial.