Lactobacillus paracasei ET-22 for Oral Pathogen Inhibition
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Solution Overview
Problem
Current probiotics lack strains that effectively promote the growth of bifidobacteria and lactic acid bacteria, enhance humoral immunity, and inhibit oral pathogens while maintaining oral health and whitening teeth, with many existing lactic acid bacteria strains failing to demonstrate these specific benefits.
Innovation Solution
The Lactobacillus paracasei ET-22 strain, deposited as CGMCC No. 15077, is identified for its ability to promote the growth of bifidobacteria and lactic acid bacteria, enhance humoral immunity, and inhibit oral pathogens by tolerating gastrointestinal stress, producing hydrogen peroxide, and being safe for food processing, making it suitable for use in various compositions such as food, pharmaceutical, and oral cleaning products.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional probiotics are used, then general intestinal health is maintained, but specific promotion of bifidobacteria and lactic acid bacteria growth is insufficient
Solution Approach 1:
The patent applies parameter changes by selecting specific strain characteristics (Lactobacillus paracasei ET-22) with optimized properties for promoting bifidobacteria and lactic acid bacteria growth. This involves changing from generic probiotic strains to a specifically characterized strain with documented efficacy in increasing beneficial bacterial populations while maintaining stability across production and storage conditions
2Object-affected harmful factors
If antibiotics are used to kill harmful bacteria, then pathogen reduction is achieved, but beneficial bacteria are also killed causing intestinal flora imbalance
Solution Approach 1:
The patent converts the harmful effect of broad-spectrum antibiotic use into a beneficial outcome by employing probiotic strains that specifically promote beneficial bacteria growth. The Lactobacillus paracasei ET-22 strain is designed to restore and maintain intestinal flora balance after antibiotic treatment by selectively enhancing bifidobacteria and lactic acid bacteria populations without requiring additional antibiotic intervention
Solution Approach 2:
The patent uses probiotic bacteria as an intermediary substance that mediates between harmful bacteria elimination and beneficial bacteria preservation. The Lactobacillus paracasei ET-22 acts as a biological mediator that creates a favorable environment for beneficial bacteria recovery while preventing pathogen recolonization, thereby restoring intestinal flora balance without direct antibiotic contact
3Reliability
If probiotics are administered to enhance immunity, then immune function is improved, but specific humoral immunity enhancement and NK cell activation are not sufficiently demonstrated
Solution Approach 1:
The patent applies parameter changes by selecting and characterizing a specific probiotic strain (Lactobacillus paracasei ET-22) with optimized immunomodulatory properties. This involves identifying strains that specifically enhance humoral immunity markers and NK cell activity, moving beyond general immune support to targeted immunological enhancement with measurable outcomes in antibody production and natural killer cell function
4Object-affected harmful factors
If lactic acid bacteria are used for oral health, then general oral hygiene is improved, but specific inhibition of oral pathogens and teeth whitening are not effectively achieved
Solution Approach 1:
The patent converts the potential harm of oral bacteria adhesion into benefit by using probiotic strains that compete with pathogens for adhesion sites. The Lactobacillus paracasei ET-22 strain is designed to occupy tooth surface and plaque niches, preventing harmful bacteria from establishing colonies while simultaneously producing substances that inhibit pathogen growth and contribute to teeth whitening through oxidative mechanisms
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Lactobacillus paracasei ET-22 significantly increases bifidobacteria and lactic acid bacteria counts, enhances humoral immunity, and effectively inhibits oral pathogens like Streptococcus mutans, Fusobacterium nucleatum, and Porphyromonas gingivalis, while being safe and effective in maintaining oral health and whitening teeth without causing antibiotic resistance or acute toxicity.
Implementation Method 1
producing hydrogen peroxide
Data Source
AI summary
The present application relates to a Lactobacillus paracasei ET-22 strain and use thereof. The present application provides a Lactobacillus paracasei ET-22 strain with a deposit number of CGMCC No. 15077, a composition containing the strain and a method of treating a subject using the strain. The present application also relates to a method for treating a subject, including administering an effective amount of the Lactobacillus paracasei ET-22 strain to a subject in need thereof for 1) whitening teeth and/or inhibiting oral pathogens; 2) adjusting balance of flora in the subject; 3) promoting growth of bifidobacteria and/or lactic acid bacteria; and/or 4) enhancing immunity of the subject.