Lactobacillus paracasei GM-080 Extracellular Vesicles for Immune Modulation
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Solution Overview
Problem
Current probiotics and their derivatives are inconsistent in modulating immune function to effectively reduce allergic reactions, and there is a need for natural substances that can regulate immunocompetence and alleviate allergic symptoms without side effects.
Innovation Solution
The use of Lactobacillus paracasei GM-080 extracellular vesicles and specific nucleotide fragments, such as those with SEQ ID NO. 7, 10, 14, 15, 16, 17, or 18, which are isolated and concentrated using a 100 kDa ultrafiltration membrane, to stimulate IFN-γ and IL-12 secretion, thereby modulating immune function and reducing allergic responses.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional probiotics are used to modulate immune function, then immune regulation is attempted, but the efficacy is inconsistent and not all probiotics have the desired function
Solution Approach 1:
The patent extracts the active immunomodulatory components (extracellular vesicles and nucleotide fragments) from the probiotic Lactobacillus paracasei GM-080. By isolating these specific functional elements rather than using whole probiotics, the invention achieves consistent and reliable immune modulation effects, resolving the inconsistency problem of conventional probiotics.
Solution Approach 2:
The patent changes the form and state of the probiotic material from whole bacteria to purified extracellular vesicles and nucleotide fragments. This parameter change (from complex biological entity to isolated molecular components) enables standardized production and consistent efficacy while maintaining the immunomodulatory function.
2Object-affected harmful factors
If antihistamines and allergy drugs are used to relieve allergic symptoms, then allergic symptoms are relieved, but certain side effects occur
Solution Approach 1:
The patent converts the harmful overactive immune response (allergy) into a beneficial controlled response by using natural immunomodulatory substances. The extracellular vesicles and nucleotide fragments from Lactobacillus paracasei GM-080 stimulate the immune system in a controlled manner to reduce allergic reactions, converting the harmful immune overreaction into a beneficial therapeutic effect without the side effects of conventional drugs.
3Object-generated harmful factors
If natural substances are used to modulate immune function, then side effects are reduced, but the efficacy to regulate immunocompetence is inconsistent
Solution Approach 1:
The patent extracts and isolates the specific active components (extracellular vesicles and nucleotide fragments) from natural probiotic sources. This extraction process concentrates the immunomodulatory activity into defined molecular forms, providing consistent and reliable efficacy while maintaining the natural, side-effect-free advantage.
Solution Approach 2:
The patent utilizes composite biological materials - extracellular vesicles containing nucleotide fragments - from Lactobacillus paracasei GM-080. These composite structures provide synergistic effects that enhance immunomodulatory efficacy while maintaining natural safety profiles.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The Lactobacillus paracasei GM-080 extracellular vesicles and nucleotide fragments effectively suppress allergic reactions and inflammation, providing a natural and effective option for immune function modulation, suitable for pharmaceutical compositions, nutritional supplements, or health foods.
Implementation Method 1
isolating the extracellular vesicle by concentration with a 100 kDa NMWL (nominal molecular weight limit) ultrafiltration membrane
Data Source
AI summary
The invention provides an extracellular vesicle and a nucleotide fragment isolated from Lactobacillus paracasei GM-080 with the deposition number BCRC 910220 and CCTCC M 204012 and use thereof. The invention also relates to a method for modulating immune function comprising administering a composition including the extracellular vesicle and the nucleotide fragment.