Lactobacillus paracasei for Inhibiting Oral Pathogens
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Solution Overview
Problem
Current solutions for preventing dental caries and periodontal diseases caused by bacteria, such as dental plaques, have not effectively utilized Lactobacillus paracasei in the oral cavity, despite its known immunomodulatory benefits and ability to inhibit bacterial growth in the gut.
Innovation Solution
A Lactobacillus paracasei-containing product, with a deposit designation of CCTCC M 206133, is developed to inhibit the growth of pathogens causing dental caries and periodontal diseases by using the strain's competitive antagonism and antimicrobial properties, which is incorporated into food, oral hygiene products, or oral treatment medicines.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If Lactobacillus paracasei is used in the oral cavity to inhibit bacterial growth, then the number of pathogenic bacteria is reduced, but the application of this strain in oral cavity has not been previously established despite its known benefits in the gut
Solution Approach 1:
The patent applies Lactobacillus paracasei, previously known only for gut health applications, to oral cavity applications. The strain demonstrates universal antimicrobial activity against multiple oral pathogens including Streptococcus mutans, Streptococcus sobrinus, and Porphyromonas gingivalis, extending its functionality from gastrointestinal to oral health contexts.
Solution Approach 2:
The patent identifies specific parameters for effective oral application of Lactobacillus paracasei, including minimum viable count requirements (≥10^6 CFU/mL for mouthwash, ≥10^8 CFU/g for chewing gum) and interaction times with pathogens. These parameter specifications enable reliable translation of the strain's gut-health properties to oral cavity applications.
2Reliability
If Lactobacillus paracasei is applied to inhibit dental caries and periodontal diseases, then the occurrence of these diseases is reduced, but the mechanism of action in oral cavity needs to be established
Solution Approach 1:
The patent converts the harmful effects of pathogenic bacteria (dental caries, periodontal disease) into beneficial outcomes by using Lactobacillus paracasei to inhibit these pathogens. The strain's antimicrobial properties, previously known for gut health, are harnessed to prevent oral diseases, turning the problem of pathogenic bacteria into an opportunity for disease prevention.
Solution Approach 2:
The patent uses Lactobacillus paracasei as an intermediary organism to mediate between the oral cavity environment and pathogenic bacteria. The strain interacts with pathogens through competitive exclusion and antimicrobial substance production, serving as a biological mediator that prevents disease without requiring direct intervention in the complex oral microbiome.
3Reliability
If Lactobacillus paracasei is used to reduce Streptococcus mutants and prevent dental plaques, then the seedbed for periodontal pathogens is reduced, but the specific application methods and dosages need to be determined
Solution Approach 1:
The patent segments the application approach by developing different oral care products (mouthwash, chewing gum) with specific Lactobacillus paracasei formulations. Each product type has optimized parameters: mouthwash requires ≥10^6 CFU/mL with 30-second contact time, while chewing gum requires ≥10^8 CFU/g with 10-minute retention time, allowing targeted delivery to different oral sites.
Solution Approach 2:
The patent employs preliminary action by having Lactobacillus paracasei pre-colonize the oral cavity before pathogenic bacteria can establish themselves. The strain is delivered through oral care products to establish a protective presence that prevents Streptococcus mutans and other pathogens from forming dental plaques and causing disease.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The product effectively reduces the number of Streptococcus mutants and other pathogenic bacteria, thereby preventing dental caries and periodontal diseases, with active and sterilized forms of Lactobacillus paracasei demonstrating significant inhibition of bacterial growth, including a 100% inhibition ratio for certain pathogens after specific interaction times.
Implementation Method 1
the strain's competitive antagonism and antimicrobial properties
Implementation Method 2
the strain's competitive antagonism and antimicrobial properties
Data Source
Figure 1
Figure 2A~2B
Figure 3A~3E
AI summary
A Lactobacillus paracasei-containing product used for inhibiting dental diseases caused by bacteria is provided, which comprises a plurality of Lactobacillus paracasei for inhibiting the growth of bacteria of dental diseases. Particularly, foods, oral hygiene products or oral treatment medicine containing the Lactobacillus paracasei when being administered or applied to a user can inhibit or reduce the number of pathogens of dental caries and periodontal diseases in oral cavity of the user, thereby achieving the efficacy of preventing dental diseases, such as dental caries and periodontal diseases.