Lactobacillus paracasei K56 for Intestinal Flora Balance
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Solution Overview
Problem
Current probiotic strains, including Lactobacilli, face challenges in maintaining viability under gastric acid and bile salt stress, and some strains exhibit antibiotic resistance, disrupting intestinal flora balance and posing health risks.
Innovation Solution
The Lactobacillus paracasei subsp. paracasei K56 strain, deposited as DSM27447, is used to prepare compositions that promote beneficial bacteria growth, inhibit harmful bacteria, and withstand simulated gastrointestinal stress, ensuring safety and stability for use in food and pharmaceutical applications.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If probiotic preparations are used to regulate intestinal flora, then beneficial bacteria growth is promoted, but harmful bacteria may proliferate and disrupt microecological balance
Solution Approach 1:
The patent uses Lactobacillus paracasei subsp. paracasei K56 as an intermediary organism that mediates the balance between beneficial and harmful bacteria. This strain directly competes with harmful bacteria for resources and space while producing substances that inhibit harmful bacterial growth, thereby regulating intestinal flora balance without causing harmful bacteria proliferation
2Object-affected harmful factors
If antibiotics are used to kill harmful bacteria, then harmful bacteria are reduced, but beneficial bacteria are also killed causing intestinal flora imbalance
Solution Approach 1:
The patent converts the harmful effect of antibiotics (killing both beneficial and harmful bacteria) into a beneficial outcome by using probiotic Lactobacillus K56 that specifically targets harmful bacteria through competitive exclusion and production of inhibitory substances. This approach eliminates harmful bacteria while preserving and even promoting beneficial bacteria, reversing the harmful effect of broad-spectrum antibiotic use
3Ease of operation
If Lactobacillus strains are used as probiotics, then intestinal flora regulation is achieved, but antibiotic resistance and toxic factors may appear posing health risks
Solution Approach 1:
The patent applies parameter changes by selecting and characterizing specific strains of Lactobacillus paracasei subsp. paracasei K56 with optimized properties. The strain was selected based on its lack of antibiotic resistance genes, absence of toxic factors, and demonstrated probiotic functionality. This selective parameter optimization ensures the probiotic is effective while safe for human consumption
4Productivity
If probiotic strains are subjected to food processing and gastrointestinal stress, then probiotic effects can be achieved, but bacterial viability may be reduced
Solution Approach 1:
The patent applies preliminary action by pre-adapting the Lactobacillus paracasei subsp. paracasei K56 strain to withstand food processing conditions and gastrointestinal stress before administration. The strain was pre-characterized for its acid and bile salt tolerance, ensuring it maintains viability through processing and gastric transit to deliver the intended probiotic effect in the intestine
Data Source
AI summary
A new use of Lactobacillus paracasei subsp. paracasei K56 capable of regulating the gastrointestinal flora balance is described. The deposit number of the Lactobacillus paracasei subsp. paracasei is DSM27447. This strain alone has the ability to significantly promote the growth of intestinal Bifidobacterium and Lactobacillus, suppress Desulfovibrio and/or Enterobacteria in the intestine, suppress Helicobacter and/or Escherichia-Shigella, and can endure a simulated in vitro gastrointestinal fluid stress environment. Experiments in mice show that this strain has no acute oral toxicity, no antibiotic resistance, and may be safely used in food processing.