Lactobacillus paracasei LT12 for Th1/Th2 Balance
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Solution Overview
Problem
Current treatments for allergy-related disorders, particularly those involving immunoregulation, are limited by the lack of effective strains of Lactobacillus species that can adequately regulate immunity and address Th-2 mediated immune responses, leading to overproduction of IL-4, IL-5, and IL-13, which contribute to allergic reactions.
Innovation Solution
A novel Lactobacillus paracasei strain, LT12, is isolated and characterized for its immunoregulating activity, capable of stimulating IFN-γ secretion and balancing Th1/Th2 activities, which is used in compositions such as food products and medicaments to regulate immunity and treat allergies.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional Lactobacillus strains are used for immunoregulation, then some immune modulation occurs, but they cannot adequately inhibit Th-2 mediated immune response and overproduction of IL-4, IL-5, and IL-13
Solution Approach 1:
The patent changes the biological parameter by introducing a novel Lactobacillus paracasei strain LT12 with specific immunoregulatory capabilities that differ from conventional strains. This strain demonstrates enhanced ability to stimulate IFN-γ secretion and regulate Th1/Th2 balance, directly addressing the insufficiency of existing strains in inhibiting Th-2 mediated immune responses
Solution Approach 2:
The patent identifies and replicates the successful immunoregulatory mechanism observed in Lactobacillus paracasei strain GM-080 (which stimulates IFN-γ secretion) by isolating and characterizing a novel strain LT12 with similar or enhanced properties, thereby copying and improving upon the proven effective approach
2Object-affected harmful factors
If hygiene hypothesis approach is taken to upregulate Th-1 response, then allergic activities are inhibited, but the mechanism requires effective immunoregulatory agents which are currently limited
Solution Approach 1:
The Lactobacillus paracasei strain LT12 serves as a self-regulating immunomodulator that naturally stimulates IFN-γ secretion and regulates Th1/Th2 balance without requiring complex external intervention. The strain's inherent metabolic and immunological properties enable it to directly counteract Th-2 mediated allergic responses, making the hygiene hypothesis approach practically implementable
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The novel Lactobacillus paracasei strain LT12 effectively stimulates the secretion of cytokines like IFN-γ, IL-1α, IL-6, IL-10, MCP-2, and TNF-α, demonstrating its ability to inhibit allergic activities and regulate immune responses, providing a novel approach to treating allergy-related disorders.
Implementation Method 1
LT12 was capable of stimulating peripheral blood mononuclear cells (PBMCs) to secret INF-γ
Implementation Method 2
The strain LT12 or its variant having immunoregulating activities and/or anti-allergic activities
Data Source
Figure 1(A)~1(E)
AI summary
An isolated Lactobacillus paracasei strain LT12 and its variant that possesses immune regulating activity and uses thereof for regulating immune responses and treating allergy.