Lactobacillus paracasei NTU 101 DNA Markers for Rapid Strain Identification
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Solution Overview
Problem
The identification of Lactobacillus paracasei subsp. paracasei NTU 101 strain using DNA molecular marker technology is incomplete due to lack of specific DNA sequence and RAPD genetic variation map, hindering rapid strain identification without culturing isolated bacteria.
Innovation Solution
Providing the nucleotide sequence of Lactobacillus paracasei subsp. paracasei NTU 101 and specific primers for PCR and RAPD processes, enabling rapid strain identification using DNA molecular markers without culturing live bacteria.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional strain identification methods are used, then accurate identification can be achieved, but time-consuming culturing processes are required
Solution Approach 1:
The patent creates a DNA copy (molecular marker) of the Lactobacillus strain that can be detected without culturing the actual bacteria. By developing specific primers that amplify and detect the strain's unique DNA sequence, the invention enables direct identification from samples, eliminating the time-consuming culturing step while maintaining identification accuracy.
Solution Approach 2:
The patent replaces the mechanical/biological culturing process with a molecular detection system. Instead of growing bacteria in culture media and observing colony characteristics, the invention uses PCR amplification and DNA hybridization techniques to detect strain-specific genetic markers, substituting a faster molecular method for the traditional microbiological approach.
2Productivity
If DNA molecular marker technology is implemented, then rapid strain identification is enabled, but specific DNA sequences and molecular markers must be developed first
Solution Approach 1:
The patent performs preliminary work by identifying and characterizing the specific DNA sequence of the Lactobacillus strain beforehand. Through genome sequencing and analysis, the invention determines unique genetic markers and develops corresponding primers in advance, so that once the molecular marker system is established, rapid identification can be performed without needing to redevelop markers for each identification task.
Solution Approach 2:
The patent changes the detection parameters from phenotypic characteristics (colony morphology, growth conditions) to genotypic parameters (DNA sequences, genetic markers). By targeting specific DNA regions with defined sequences and developing primers with optimized binding characteristics, the invention creates a molecular marker system that is both rapid to implement and highly specific to the target strain.
3Measurement precision
If specific primers for PCR and RAPD are designed, then accurate and rapid identification is achieved, but primer design and validation processes are required
Solution Approach 1:
The patent extracts the specific DNA sequence information from the Lactobacillus strain genome and isolates the most suitable regions for marker development. By selecting specific genomic regions that are unique to the strain and designing primers targeting only those regions, the invention achieves high identification specificity while reducing the complexity of primer validation, as the primers are designed to amplify only the intended target without cross-reacting with other sequences.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Facilitates rapid and accurate identification of Lactobacillus paracasei subsp. paracasei NTU 101 using DNA molecular markers, enhancing the ability to utilize this strain for health applications such as improving intestinal flora and reducing gastric mucosal lesions.
Implementation Method 1
the nucleotide sequence of the Lactobacillus paracasei subsp. paracasei NTU 101 can be formed by treating the RAPD (Random Amplification of Polymorphic DNA) and the PCR (Polymerase Chain Reaction) process to a plurality of specific primers
Implementation Method 2
the nucleotide sequence of the Lactobacillus paracasei subsp. paracasei NTU 101 can be formed by treating the RAPD (Random Amplification of Polymorphic DNA) and the PCR (Polymerase Chain Reaction) process to a plurality of specific primers
Data Source
AI summary
The present invention relates to a lactobacillus mutant, a nucleotide sequence for lactobacillus mutant, and primers for nucleotide sequence of lactobacillus mutant. The lactobacillus mutant is Lactobacillus paracasei subsp. paracasei NTU 101 having the nucleotide sequence of SEQ ID NO 1, and deposited with Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ, Germany) on November 18, 2013, wherein the accession number of Lactobacillus paracasei subsp. paracasei NTU 101 is DSM 28047. Moreover, a nucleotide sequence for NTU 101 and the primers for the nucleotide sequence are also proposed for facilitating the person skilled in Lactobacillus filed capable of carrying out the strain identification of the NTU 101 according to the present invention. Moreover, the person skilled in Lactobacillus filed can also rapidly complete the strain identification of the NTU 101 by using DNA molecular marker technology, without culturing any isolated Lactobacillus strain or live Lactobacillus bacteria.