Lactobacillus paracasei SG96 for Antibiotic-Free Animal Feed

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Solution Overview

Problem

The widespread use of antibiotics in animal feeds has led to the emergence of drug-resistant bacteria, posing health risks to humans and animals, and there is a need for alternative solutions to promote animal health and safety in meat products without relying on antibiotics.

Innovation Solution

A novel Lactobacillus paracasei subsp. paracasei SG96 strain is identified and developed, which is shown to inhibit pathogenic bacteria such as Escherichia coli and Salmonella typhimurium, and is formulated into a bacteriostatic composition for use in animal feeds and human food products to enhance pathogenic bacteria-resistant abilities.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If antibiotics are added to animal feeds to promote growth and prevent disease, then animal health and growth efficiency are improved, but drug-resistant bacteria emerge and human health is compromised

Engineering Contradiction:
Improveanimal healthVSAvoiddrug-resistant bacteria
Core Design Contradiction:
ReliabilityVSObject-generated harmful factors

Solution Approach 1:

The patent converts the harmful effect of antibiotics into a beneficial alternative by using probiotic bacteria (Lactobacillus paracasei SG96) that naturally inhibit pathogenic bacteria. The probiotic strain produces bacteriocins and competes for resources, achieving disease prevention without selecting for drug-resistant strains. This transforms the harmful dependency on antibiotics into a beneficial microbial ecosystem approach.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The patent introduces an intermediary substance - the probiotic bacteria Lactobacillus paracasei SG96 - that mediates between the need for disease prevention and the avoidance of drug resistance. This intermediary organism provides protective effects against pathogenic bacteria through natural mechanisms, serving as a bridge between animal health requirements and public health safety.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Reliability

If antibiotics are used to control bacterial infections in animals, then disease treatment effectiveness is improved, but adverse effects on human health increase through residual antibiotics in food products

Engineering Contradiction:
Improvedisease treatment effectivenessVSAvoidadverse effects on human health
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent converts the harmful residual antibiotics in food products into a beneficial probiotic alternative. Instead of leaving harmful drug residues in meat, eggs, and milk, the probiotic bacteria provide treatment effectiveness through natural bacterial competition and toxin production, eliminating the source of human health adverse effects while maintaining disease control capability.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The patent replaces long-lasting antibiotic residues in the food chain with a temporary but effective probiotic intervention. The probiotic bacteria perform their protective function during the animal's growth period and then degrade naturally, leaving no persistent harmful substances in the food products, thus eliminating long-term human health concerns.

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

3Object-affected harmful factors

If antibiotics are forbidden in animal feeds to reduce drug resistance, then human health safety is improved, but animal growth promotion and disease prevention capabilities deteriorate

Engineering Contradiction:
Improvehuman health safetyVSAvoiddisease prevention capability
Core Design Contradiction:
Object-affected harmful factorsVSReliability

Solution Approach 1:

The patent changes the fundamental parameter of disease prevention from chemical (antibiotics) to biological (probiotic bacteria). By switching from pharmacological intervention to microbial ecology-based prevention, the system maintains disease control effectiveness while eliminating drug resistance issues and ensuring human health safety. The probiotic strain Lactobacillus paracasei SG96 provides prevention through natural mechanisms including bacteriocin production and competitive exclusion.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent substitutes the chemical-mechanical action of antibiotics with a biological system - the probiotic bacteria. This replacement uses biological mechanisms such as bacterial competition, phage production, and immune modulation to achieve disease prevention, eliminating the need for chemical antibiotics and their associated resistance problems while maintaining preventive effectiveness.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The Lactobacillus paracasei subsp. paracasei SG96 strain effectively suppresses pathogenic bacteria growth, promotes weight gain, improves feed conversion rates, and exhibits better anti-diarrhea effects in un-weaned piglets, demonstrating its potential as a safer alternative to antibiotics in animal husbandry and human consumption.

Implementation Method 1

a novel Lactobacillus paracasei subsp. paracasei SG96 strain, and in particular, to a novel Lactobacillus paracasei subsp. paracasei microbial strain that can inhibits pathogenic bacteria such as Escherichia coli, Salmonella typhimurium

Methodology Applied
Scientific EffectBacteriocin production:

Implementation Method 2

Lactic acid bacteria can maintain and restore the balance of bacterial colonies in the digestive tract of poultry and livestock, smooth away the stress of poultry and livestock, enhance the immunological and disease-resistant abilities of poultry and livestock

Methodology Applied
Scientific EffectLactic acid bacteria fermentation: Fermentation

Data Source

PatentEP2166083B1Novel Lactobacillus paracasei subsp. paracasei SG96, a bacteriostatic composition containing the same and use thereof
Publication Date: 2010.12.22 SYNGEN BIOTECH CO LTD
  • EP2166083B1 patent drawingFigure 1A~1B
  • EP2166083B1 patent drawingFigure 2
  • EP2166083B1 patent drawingFigure 3

AI summary

A novel Lactobacillus paracasei subsp. paracasei SG96, a bacteriostatic composition containing the same and use thereof, comprise a novel Lactobacillus paracasei subsp. paracasei SG96 strain, wherein said strain had been deposited in China General Microbiological Culture Collection Center, CGMCC, accession number: CGMCC 2697, characterized in that said strain can inhibit the growth of pathogenic bacteria such as Escherichia coli, Salmonella typhimurium and the like, and even has an effect of killing bacterial; and a bacteriostatic composition containing said Lactobacillus paracasei subsp. paracasei SG96 strain, characterized in that said composition can be used as an additive in animal drinking water, as an additive in animal feed, as an medical composition for animal and mankind, as an additive in food product, as an additive in beverage, and as a food product, beverage, and healthy food products., and the like.