Lactococcus lactis SOD Enzymes Neutralizing ROS in Gut Inflammation
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Solution Overview
Problem
Current therapies are inadequate in addressing the oxidative stress and inflammation caused by reactive oxygen species in inflammatory bowel diseases, such as ulcerative colitis and Crohn's disease, which lead to tissue damage and chronic inflammation in the gastrointestinal tract.
Innovation Solution
The use of superoxide dismutase (SOD) enzymes derived from Lactococcus lactis bacteria, specifically the CNCM I-1631 strain, to neutralize reactive oxygen species and reduce oxidative stress, administered as a composition for therapeutic or preventative purposes, either as a medicament or in functional foods, to treat inflammatory conditions and prevent relapse.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional therapies are used to treat inflammatory bowel disease, then some symptoms may be managed, but they are inadequate in addressing oxidative stress and inflammation caused by reactive oxygen species, leading to persistent tissue damage and chronic inflammation
Solution Approach 1:
The patent applies this principle by using lactic acid bacteria that naturally produce superoxide dismutase enzyme to convert the harmful oxidative stress environment into a beneficial therapeutic effect. The bacteria are engineered to overproduce SOD enzyme, which then neutralizes reactive oxygen species that would otherwise cause tissue damage, effectively converting the harmful oxidative environment into a protective antioxidant therapy
Solution Approach 2:
The patent uses lactic acid bacteria as an intermediary organism that delivers the superoxide dismutase enzyme to the gastrointestinal tract. These bacteria serve as a living vehicle that produces and releases the antioxidant enzyme directly at the site of inflammation, acting as a mediator between the therapeutic goal (reducing oxidative stress) and the affected tissue
2Reliability
If reactive oxygen species accumulate in the gastrointestinal tract, then pro-inflammatory signaling is activated and transcription factors are up-regulated, but this leads to worsened inflammation and tissue injury
Solution Approach 1:
The patent converts the harmful pro-inflammatory signaling pathway into a beneficial anti-inflammatory effect by introducing lactic acid bacteria that produce superoxide dismutase. The enzyme neutralizes reactive oxygen species before they can activate pro-inflammatory transcription factors, thereby converting the inflammatory response into an anti-inflammatory therapeutic effect
Solution Approach 2:
The patent applies preliminary anti-action by having lactic acid bacteria produce superoxide dismutase enzyme in advance to prevent the activation of pro-inflammatory signaling pathways. The enzyme is present before reactive oxygen species can cause significant damage, neutralizing them preemptively and preventing the cascade of inflammatory responses
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The SOD enzymes effectively reduce ROS-induced damage and oxidative stress in the gastrointestinal tract, improving symptoms and inducing remission in inflammatory bowel diseases, as demonstrated in murine models, suggesting a potential treatment for inflammatory bowel disease and related conditions.
Implementation Method 1
The use of superoxide dismutase (SOD) enzymes derived from Lactococcus lactis bacteria, specifically the CNCM I-1631 strain, to neutralize reactive oxygen species
Implementation Method 2
superoxide dismutase (SOD) enzymes such as superoxide dismutase A enzymes... having particularly desirable properties (e.g., antioxidant properties)
Data Source
AI summary
Disclosed herein are compositions and methods for providing an antioxidant effect to a subject, e.g., modulating or otherwise improving oxidative stress pathways and/or neutralizing or reducing damaging reactive oxygen species (ROS).