Lactose-Free Dairy Process Using Enzyme Recycling

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Solution Overview

Problem

Existing methods for producing lactose-free dairy products are inefficient due to long processing times and the presence of residual enzymes in the final products, which affects profitability and product quality.

Innovation Solution

A process involving ultrafiltration, enzymatic degradation of lactose in a reactor, followed by nanofiltration, and recycling of unreacted lactose and enzyme, ensuring complete lactose removal and enzyme inactivation, allowing for continuous operation and significantly reduced processing time.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Manufacturing precision

If conventional enzymatic degradation methods are used to break down lactose, then lactose is hydrolyzed into glucose and galactose, but the process requires long standing times and leaves residual enzymes in the product

Engineering Contradiction:
Improvelactose removal completenessVSAvoidprocessing time
Core Design Contradiction:
Manufacturing precisionVSLoss of time

Solution Approach 1:

The patent extracts and removes residual enzymes from the reaction mixture using filtration or centrifugation steps after enzymatic hydrolysis. This separation step eliminates the harmful residual enzymes while preserving the hydrolyzed sugars, resolving the contradiction between complete lactose removal and short processing time by adding a targeted removal step rather than extending the hydrolysis time

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent introduces an intermediary separation step (filtration/centrifugation) between the enzymatic reaction and the final product. This intermediary process removes the enzyme catalyst from the mixture, allowing the reaction to be completed quickly without the time-consuming extended standing period, thus resolving the time-quality contradiction

Inventive Principle:
Principle #24Intermediary (Mediator)

2Manufacturing precision

If conventional enzymatic degradation methods are used, then lactose is broken down, but enzymes remain in the final product affecting quality and profitability

Engineering Contradiction:
Improvelactose-free product qualityVSAvoidprocess simplicity
Core Design Contradiction:
Manufacturing precisionVSEase of manufacture

Solution Approach 1:

The patent applies extraction by removing residual enzymes through filtration or centrifugation after the enzymatic reaction. This targeted removal ensures high product quality (lactose-free with no residual enzymes) while maintaining relatively simple processing by using standard separation techniques rather than complex purification systems

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent employs self-service by using the enzyme's own catalytic action to complete the lactose breakdown, then allowing simple physical separation methods to remove the enzyme. The system uses natural enzymatic activity followed by passive separation, avoiding the need for complex additional processing steps to achieve high-quality results

Inventive Principle:
Principle #25Self-service

3Manufacturing precision

If lactase is added in sufficient amounts for complete lactose degradation, then lactose is fully broken down, but enzyme usage costs increase

Engineering Contradiction:
Improvecomplete lactose hydrolysisVSAvoidenzyme consumption
Core Design Contradiction:
Manufacturing precisionVSLoss of substance

Solution Approach 1:

The patent implements continuous enzymatic reaction with controlled addition of lactase, maintaining optimal enzyme concentration throughout the process rather than adding large excess amounts. The reaction proceeds continuously with sufficient but not excessive enzyme, completing lactose hydrolysis efficiently while minimizing enzyme consumption and cost

Inventive Principle:
Principle #20Continuity of useful action

Solution Approach 2:

The patent optimizes reaction parameters (temperature, pH, residence time) to maximize enzyme efficiency. By controlling these parameters, the process achieves complete lactose hydrolysis with minimal enzyme dosage, reducing enzyme consumption costs while maintaining high manufacturing precision

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This process results in lactose-free products with less than 0.1% lactose content and no residual enzymes, enhancing throughput and profitability by minimizing enzyme usage and enabling both continuous and discontinuous operation.

Implementation Method 1

the permeate P1 is fed to the enzymatic degradation of the lactose contained therein adding a sufficient amount of lactase

Methodology Applied
Scientific EffectEnzymatic hydrolysis: Enzyme

Implementation Method 2

skimmed milk is subjected to ultrafiltration or a combination of dia- and ultrafiltration

Methodology Applied
Scientific EffectUltrafiltration: Semipermeable Membrane

Implementation Method 3

subjecting the resulting reaction mixture to nanofiltration

Methodology Applied
Scientific EffectNanofiltration: Semipermeable Membrane

Data Source

PatentEP2907393B1Milk products free of lactose
Publication Date: 2018.07.25 DMK DEUT MILCHKONTOR
  • EP2907393B1 patent drawingFigure 1
  • EP2907393B1 patent drawing

AI summary

A process for the production of lactose-free dairy products is proposed, in which (a) skimmed milk is subjected to ultrafiltration or a combination of diafiltration and ultrafiltration, (b) the lactose-free retentate R1 is directed into a storage container and the lactose-containing permeate P1 into an enzyme reactor, (c) a sufficient amount of lactase for the enzymatic degradation of the lactose contained therein is added to the permeate P1, (d) the reaction mixture thus obtained is subjected to nanofiltration, (e) the lactose-free permeate P2 is conveyed into the storage container and mixed with the lactose-free retentate R2, and (f) the retentate R2, which contains unreacted lactose and enzyme, is returned to the enzyme reactor.