Lactuca CMS Plant Low Temperature Growth via Asymmetric Protoplast Fusion
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Solution Overview
Problem
The conventional cytoplasmic male sterile (CMS) plant of the genus Lactuca exhibits reduced low temperature growth ability, which is an unfavorable trait limiting its industrial usability and seed production efficiency.
Innovation Solution
Improvement of the CMS plant's mitochondrial genome through asymmetric protoplast fusion using a CMS plant as a cytoplasm donor and a plant with normal cytoplasm as an acceptor, incorporating DNA from Helianthus to enhance low temperature growth ability, allowing for efficient production of F1 seeds with improved traits.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional CMS plant cytoplasm is used, then male sterility is achieved, but low temperature growth ability is reduced
Solution Approach 1:
The invention separates the male sterility trait from the cytoplasmic background by using asymmetric protoplast fusion to transfer only the male sterility determinant to a different cytoplasmic environment, thereby isolating the unwanted low temperature growth defect from the desired male sterility trait
Solution Approach 2:
A third plant species serves as an intermediary donor in the asymmetric protoplast fusion process, providing a compatible cytoplasmic background that supports both male sterility expression and improved low temperature growth ability without direct crossing between the original CMS plant and the target plant
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified CMS plant demonstrates low temperature growth ability comparable to plants with normal cytoplasm, enabling efficient production of high-quality F1 seeds with improved marketability and environmental adaptability.
Implementation Method 1
Improvement of the CMS plant's mitochondrial genome through asymmetric protoplast fusion using a CMS plant as a cytoplasm donor and a plant with normal cytoplasm as an acceptor
Data Source
AI summary
A cytoplasmic male sterile plant of the genus Lactuca is provided having low temperature growth ability comparable to that of a plant of the genus Lactuca with a normal cytoplasm, or a progeny thereof. For example, it is possible to alleviate a reduction in the growth ability at low temperature observed in previous CMS plants of the genus Lactuca and provide a CMS plant of the genus Lactuca having low temperature growth ability.