LAMP Polynucleotide Primers for Rapid SARS-CoV-2 Detection
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Solution Overview
Problem
There is an urgent need for a rapid, affordable, and efficient point-of-care diagnostic platform to detect the SARS-CoV-2 virus, which has been hindered by the inability to test members of the public effectively during the COVID-19 pandemic.
Innovation Solution
The development of a composition comprising a set of polynucleotides and a probe, specifically designed for loop-mediated isothermal amplification (LAMP), which targets specific genes in the SARS-CoV-2 viral genome, allowing for rapid and accurate detection of the virus.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Speed
If conventional diagnostic methods are used for SARS-CoV-2 detection, then detection accuracy can be maintained, but the testing speed and accessibility are insufficient
Solution Approach 1:
The patent changes the operational parameters of nucleic acid amplification by using isothermal conditions (constant temperature around 60-65°C) instead of cyclic temperature changes in PCR. This parameter change enables rapid amplification without complex thermal cycling equipment, achieving both fast detection speed and reliable results through the LAMP amplification method with specifically designed primers and probes
Solution Approach 2:
The patent replaces the mechanical thermal cycling system of conventional PCR with an isothermal chemical-biological system. By substituting the mechanical temperature cycling apparatus with a simple heating block or even body-temperature incubation, the system achieves rapid detection while maintaining reliability through the use of temperature-independent LAMP amplification chemistry
2Measurement precision
If complex diagnostic equipment is used, then detection accuracy improves, but device complexity and cost increase
Solution Approach 1:
The patent extracts and removes the complex thermal cycling apparatus from the diagnostic system, retaining only the essential isothermal amplification chemistry and simple heating mechanism. By taking out the unnecessary complex equipment while keeping the core detection function, the system achieves high detection precision through specific primer-probe designs without requiring sophisticated instrumentation
Solution Approach 2:
The patent employs disposable, inexpensive reagent mixes containing pre-optimized LAMP primers and molecular beacon probes that can be discarded after single use. This approach replaces expensive, complex reusable equipment with cheap, single-use consumables that maintain high detection precision through carefully designed nucleic acid sequences while eliminating the need for maintaining complex diagnostic devices
3Measurement precision
If specific gene targeting is implemented, then detection accuracy for SARS-CoV-2 improves, but the ability to distinguish from related coronaviruses becomes more challenging
Solution Approach 1:
The patent applies local quality by designing primers and molecular beacon probes that target highly specific local regions (unique sequences) within the SARS-CoV-2 genome, particularly in the N gene and other conserved regions. By focusing specificity on particular local sequence features rather than broad genomic regions, the system achieves accurate SARS-CoV-2 detection while the combination of multiple targeted sites provides differentiation capability from related coronaviruses through pattern recognition
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This solution enables rapid and affordable detection of SARS-CoV-2, facilitating timely intervention and public health management during the pandemic, with the ability to distinguish between SARS-CoV-2 and closely related human coronaviruses.
Implementation Method 1
The compositions and methods disclosed herein provide primers and probes for the detection of the SARS-CoV-2 RNA virus using loop-mediated isothermal amplification
Implementation Method 2
The present invention encompasses, in some embodiments, a composition comprising a set of polynucleotides selected from the group consisting of Set-1 through Set-17. In some embodiments, the composition further comprises a probe
Data Source
AI summary
Disclosed herein are primers and probes related to the detection of SARS-CoV-2 via nucleic acid amplification testing (NAAT), for example to amplify and determine the presence of SARS-CoV-2 in test samples and/or to diagnose Covid-19. Specifically, the present disclosure describes primers and probes that bind to the N gene, ORF1ab, or E gene of SARS-CoV-2 coronavirus for detection via loop mediated isothermal amplification (LAMP) and molecular beacon hybridization.