LAMP Temperature Detection Device with Integrated Sensor
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Solution Overview
Problem
Traditional temperature detection methods in LAMP processes are inaccurate due to non-uniform heat distribution and require invasive measurements, which disrupt the closed environment needed for sample amplification.
Innovation Solution
A temperature detection device with a temperature measurement mechanism, heat conduction pad, heating layer, and thermal insulation layer that allows direct temperature measurement within the microfluidic chip's working chamber, using ceramic heating plates and temperature sensors to maintain precise temperature control through a PID feedback algorithm.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a thermometer is used for temperature measurement in LAMP, then temperature data can be obtained, but the measurement accuracy is insufficient due to non-uniform heat distribution and the invasive measurement disrupts the closed environment
Solution Approach 1:
The patent introduces a temperature sensor as an intermediary element that can be integrated into the heating block structure. This sensor acts as a mediator between the heating system and the control system, allowing temperature measurement without requiring external invasive measurement. The sensor is positioned to directly contact the sample chamber through the heating block, enabling accurate temperature detection while maintaining the closed environment of the LAMP reaction system.
Solution Approach 2:
The patent combines the temperature measurement function with the heating block structure by integrating the temperature sensor directly into the heating block. This merging of measurement and heating functions allows the sensor to be positioned optimally for accurate temperature detection while the heating block maintains the closed reaction environment. The integrated design eliminates the need for separate invasive measurement procedures.
2Measurement precision
If a thermometer is inserted into the temperature measurement chamber for measurement, then temperature values can be read, but the closed environment for sample amplification is compromised
Solution Approach 1:
The temperature sensor serves as an intermediary that bridges the heating block and the control system. It is positioned within the heating block structure to directly monitor the temperature of the sample chamber without requiring insertion into the reaction mixture. This intermediary positioning allows continuous temperature data collection while the sealed LAMP reaction proceeds undisturbed in the closed environment.
Solution Approach 2:
The patent transitions from external invasive measurement to internal integrated measurement by embedding the temperature sensor within the heating block structure. This dimensional repositioning allows the sensor to measure temperature from within the heating system itself, eliminating the need to insert measurement devices into the reaction chamber and thereby preserving the closed environment integrity while maintaining reliable temperature monitoring.
3Temperature
If heating sources are applied to the microfluidic chip, then temperature control is achieved, but heat loss to the environment reduces efficiency
Solution Approach 1:
The patent applies heating sources directly to the specific regions of the microfluidic chip where temperature control is needed, rather than heating the entire system uniformly. The heating elements are positioned in thermal contact with the chip at the reaction chambers, providing localized heating that achieves precise temperature control while minimizing heat loss to surrounding areas. This localized approach reduces energy waste by concentrating thermal energy only where required for the LAMP reaction.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate and stable temperature detection and control within the microfluidic chip, ensuring efficient LAMP processes by preventing heat loss and maintaining consistent heating regions.
Implementation Method 1
at least two heating sources are arranged on the heating layer; heating regions corresponding to various heating sources are formed on the surface of the heat conduction pad
Implementation Method 2
heating regions corresponding to various heating sources are formed on the surface of the heat conduction pad; the heats generated by the heating sources in the heating layer are all transmitted to the corresponding heating regions through the heat conduction pad
Implementation Method 3
the thermal insulation layer prevents the heating layer from downwards heat transmitting such that the heats generated by the heating sources in the heating layer are all transmitted to the corresponding heating regions through the heat conduction pad
Implementation Method 4
the temperature measurement mechanism enters the corresponding temperature measurement chamber to collect corresponding working chamber temperature data
Data Source
AI summary
The present disclosure belongs to the technical field of temperature detection devices for microfluidic chips, specifically relates to a temperature detection device and method in loop mediated isothermal amplification (LAMP). The temperature detection device in the LAMP includes a temperature measurement mechanism, a heat conduction pad, a heating layer, and a thermal insulation layer which are disposed in sequence from top to bottom; the temperature measurement mechanism enters a corresponding temperature measurement chamber to collect corresponding working chamber temperature data, that is, the heating layer adjusts, according to the corresponding working chamber temperature data, heat generated by a corresponding heating source; the thermal insulation layer prevents the heating layer from downwards transmitting the heat such that the heats generated by the heating sources in the heating layer are all transmitted to corresponding heating regions through the heat conduction pad. In the present disclosure, the temperature measurement mechanism feeds back the working chamber temperature data serving as a temperature stabilization basis of the microfluidic chip, so that the temperature in a LAMP process can be accurately detected, and stable and efficient LAMP is guaranteed.