Laser Scanning Imaging with Dynamic Clocking for Flexible ROI Sampling
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Solution Overview
Problem
Existing laser scanning imaging systems are limited by fixed clock signals generated by hardware clock boards, resulting in a small and fixed imaging area with inflexible resolution, unable to accommodate changes in scanning range.
Innovation Solution
A method that generates a clock signal based on galvanometer scanning synchronization signals and parameter information related to the effective area, allowing flexible alteration of the clock signal to accommodate changes in the imaging area, enabling accurate fluorescence image capture.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If a hardware clock board is used to generate a clock signal from the galvanometer scanning synchronization signal, then the clock signal can be generated for sampling the fluorescence signal, but the imaging area becomes fixed and the resolution cannot be flexibly adjusted
Solution Approach 1:
The patent replaces the hardware clock board (mechanical/electronic system) with a software-based clock signal generation method. The processor generates the clock signal by processing the galvanometer scanning synchronization signal according to preset effective area parameters, eliminating the need for fixed hardware clock generation and enabling flexible adjustment of imaging area and resolution through software configuration.
2Area of stationary object
If the clock signal is generated only in a fixed portion of the resonant galvanometer motion, then the clock signal generation is simplified, but the imaging range becomes limited and the imaging position is fixed
Solution Approach 1:
The patent makes the clock signal generation dynamic by allowing the effective area parameters (which determine the imaging range) to be adjusted according to different scanning requirements. The processor dynamically generates the clock signal based on the current effective area parameters, enabling the imaging area and position to be flexibly changed without complicating the clock signal generation process.
3Measurement precision
If the imaging area is changed by amplification processing, then the displayed area can be adjusted, but the actual resolution of the fluorescence image cannot be increased
Solution Approach 1:
The patent performs preliminary action by pre-setting the effective area parameters (including imaging range and resolution requirements) before fluorescence signal acquisition. The processor generates the clock signal and controls the scanning and sampling processes according to these preset parameters, ensuring that the actual resolution is determined during the acquisition process itself rather than through post-acquisition amplification, thereby maintaining both high resolution and acquisition efficiency.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables flexible adjustment of the imaging area and resolution to meet user requirements, providing accurate fluorescence images that satisfy specific scanning needs.
Implementation Method 1
the objective lens focuses the laser beam on the biological tissues, the irradiated biological tissues generate fluorescence information
Data Source
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AI summary
A laser scanning imaging method, a system, a storage medium, and a computer program. The laser scanning imaging method comprises: determining parameter information related to an effective area, where the effective area comprises an imaging area satisfying a preset condition (S101); receiving a galvanometer scanning synchronization signal generated by a driving unit (S102); generating a clock signal based on the galvanometer scanning synchronization signal and the parameter information related to the effective area (S103); and sampling, according to the clock signal, a fluorescence signal received through galvanometer scanning and obtaining fluorescence image information of the effective area (S104). By means of the method, the generated clock signal can be flexibly changed along with the change of the range of the effective area, thereby obtaining the fluorescence image of the effective area, and presenting, for a user, a scanning imaging result meeting requirements.