Lateral Flow Device for Acute Aortic Dissection Biomarker Detection
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Solution Overview
Problem
Current diagnostic methods for acute aortic dissection (AAD) are delayed due to confusion with cardiac infarction symptoms, and there is a need for a rapid, reliable, and precise test to detect proteins released during AAD events in bodily fluids.
Innovation Solution
A lateral flow device with a strip enabling capillary flow, a sample pad, a conjugate pad with protein antibody binding to AAD-proteins conjugated to detection agents, and detection bands indicating protein presence through visible color changes, specifically designed for detecting biomarkers like HMGI-C, aggrecan, and D-dimer in bodily fluids.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If current diagnostic methods are used for acute aortic dissection, then diagnosis can be made, but diagnosis is delayed due to confusion with cardiac infarction symptoms
Solution Approach 1:
The invention segments the diagnostic process by using a lateral flow device that specifically detects AAD-related proteins (HMGI-C, aggrecan, D-dimer) separately from general cardiac markers. This segmentation allows rapid differentiation between AAD and cardiac infarction within minutes, eliminating the time delay caused by symptomatic confusion while maintaining high diagnostic accuracy through targeted biomarker detection.
Solution Approach 2:
The lateral flow device performs preliminary detection of AAD-specific proteins immediately upon sample application, before more complex confirmatory tests are needed. By detecting multiple AAD biomarkers simultaneously in a single rapid test, the device provides preliminary diagnostic information that guides subsequent medical decision-making, significantly reducing overall diagnosis time.
2Loss of time
If a rapid test is developed for AAD detection, then diagnosis time is reduced, but test complexity increases
Solution Approach 1:
The lateral flow device achieves multi-functionality by simultaneously detecting multiple AAD biomarkers (HMGI-C, aggrecan, D-dimer) using a single integrated strip format. This universal approach allows the device to provide comprehensive AAD diagnostic information in one rapid test, reducing detection time while avoiding the need for multiple separate complex tests.
Solution Approach 2:
The invention uses a simplified lateral flow format that copies the essential functionality of complex immunoassay systems but in a much simpler, rapid-format device. By adapting proven immunoassay principles to a lateral flow platform with pre-coated antibodies and visual readout, the device achieves rapid detection without requiring complex instrumentation or multiple test components.
3Productivity
If lateral flow device is used for detecting AAD-proteins, then rapid detection is achieved, but detection precision must be maintained
Solution Approach 1:
The lateral flow device applies local quality by using different antibody coatings at specific locations on the strip for detecting different AAD biomarkers. Each test line is locally optimized with high-affinity antibodies specific to HMGI-C, aggrecan, or D-dimer, ensuring precise detection of each protein while maintaining rapid overall detection speed through parallel processing.
Solution Approach 2:
The device uses color change at detection lines as a visual indicator of protein presence, providing immediate qualitative results. The intensity and presence of color changes at different test lines correspond to the detection of specific AAD biomarkers, enabling rapid interpretation of results while maintaining detection precision through the specificity of antibody-antigen interactions that produce the color signal.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device provides a rapid and reliable detection of AAD-proteins in bodily fluids, facilitating timely diagnosis and improving patient outcomes by indicating protein presence through visible color changes, thereby aiding in the early identification of acute aortic dissection.
Implementation Method 1
a strip formed of a material enabling capillary flow of fluid along a portion of the strip
Implementation Method 2
a conjugate comprising a protein antibody binding to said AAD-protein
Implementation Method 3
the presence of said AAD-protein in the sample is indicated by a visible color change
Data Source
AI summary
The invention relates to a lateral flow device for the detection of at least one protein (AAD-protein) released during an event leading to an acute aortic syndrome/acute aortic dissection (AAS/AAD) in a sample of bodily fluid of a human/patient, the device comprising a strip, a sample pad for receiving the sample of the bodily fluid, a first conjugate pad with a conjugate comprising a protein antibody binding to said AAD-protein and a first detection band comprising a first conjugate-binding protein so that the presence of said AAD-protein in the sample is indicated by a visible color change, wherein the AAD-protein is selected from a group of biomarkers for AAS/AAD including D-dimer smooth muscle actin myosin isozyme CK-MB and nucleic acid component (DISAMIN). In addition, the invention relates in other aspects also to a method for the detection of an AAD-protein in the blood of a patient using the lateral flow device according to the invention, a kit comprising this device as well as a method for determination of the presence of AAD in a patient using an immunoassay.