Lateral Flow Immunoassay for Ammonia Oxidation Gene Detection
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Solution Overview
Problem
Current methods for analyzing microorganisms in anammox bioreactors are costly and require sophisticated equipment, leading to inefficient operation and increased costs due to the inability to accurately control the reactor in real-time.
Innovation Solution
A rapid detection method using a lateral flow immunoassay test strip that amplifies ammonia oxidation functional genes via PCR, allowing for real-time analysis of bacterial species in wastewater treatment, with results interpretable by naked eye within 15 minutes, using biotin and digoxigenin-modified primers and a chromogenic complex of nano colloidal gold particles and antibodies.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If molecular biological detection techniques (qPCR, microarray chip, NGS) are used to accurately analyze microorganisms in the bioreactor, then measurement precision is improved, but device complexity and cost increase
Solution Approach 1:
The patent employs lateral flow assay test strips as disposable, low-cost detection tools instead of expensive, complex molecular biology equipment. The test strips can be easily discarded after use, eliminating the need for sophisticated instruments while maintaining adequate detection capability for monitoring ammonia oxidation functional genes in the bioreactor.
Solution Approach 2:
The patent replaces complex molecular biological detection systems with a simplified lateral flow assay system that uses visual color changes on a test strip. This substitution eliminates the need for sophisticated equipment while providing sufficient measurement precision for operational monitoring of the bioreactor.
2Measurement precision
If molecular biological detection techniques are used to accurately analyze microorganisms, then measurement precision is improved, but loss of time increases due to equipment requirements
Solution Approach 1:
The lateral flow assay test strips provide rapid results within minutes, eliminating the time-consuming procedures required for molecular biology techniques. The disposable nature of the test strips allows for quick, point-of-use detection without the lengthy sample processing and equipment calibration required for qPCR, microarray, or NGS systems.
3Reliability
If physical and chemical sewage treatment methods are used to treat high concentrations of ammonia nitrogen, then treatment effectiveness is improved, but loss of energy increases due to high treatment costs
Solution Approach 1:
The patent implements real-time monitoring of ammonia oxidation functional genes in the bioreactor using lateral flow assay test strips. This feedback mechanism allows operators to monitor the biological treatment process continuously, adjust operational parameters dynamically, and optimize the biological treatment effectiveness, thereby reducing energy consumption and treatment costs compared to purely physical and chemical methods.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables immediate, cost-effective analysis of bacterial phases in bioreactors, reducing operating costs and preventing reactor paralysis by providing real-time information on microorganism activity, thus promoting the practical application of anammox bioreactors.
Implementation Method 1
The absorbent pad is used to provide a capillary attraction and collect the remaining sample
Implementation Method 2
the chromogenic substance is a complex of nano colloidal gold particles and a primary antibody
Data Source
AI summary
The invention provides a detection method of ammonia oxidation functional gene in water treatment, including the following steps. For a sample to be detected, ammonia oxidation functional genes are amplified by PCR, and primers used for PCR are modified by attaching biotin and digoxigenin to forward and reverse 5′ ends respectively. Next, on the basis of immunochemistry, antibodies are used for biorecognition, and nano colloidal gold particles are used as chromogenic substances. The sample is placed on a sample pad of a lateral flow immunoassay test strip, and the sample flows from the sample pad to an absorbent pad. Color development on both the test and control lines when the sample flows through a nitrocellulose membrane indicates a positive result; color development solely on the control line indicates a negative result and no color development indicates an invalid test.