Lateral Flow Assay Device with Flow Bridging Structure

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Solution Overview

Problem

Lateral flow assay devices face challenges in optimizing sample flow characteristics, leading to sample wastage, inadequate mixing of samples and reagents, and prolonged assay times, while the hydrophilic foil cover can hinder fluid flow and cause contamination.

Innovation Solution

A lateral flow device design featuring a substrate with projections for capillary flow, a hydrophilic foil or tape cover with a peripheral edge, and a flow bridging structure, such as a groove or bar, at the entrance of the absorbing zone to promote fluid flow and prevent wicking along the cover's edge, allowing for adjustable flow times and reduced sample volumes.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If a hydrophilic foil cover is placed over the absorbing zone to improve sample wicking, then the wicking performance is improved, but the cover can hinder fluid flow and cause contamination

Engineering Contradiction:
Improvewicking performanceVSAvoidflow hindrance and contamination
Core Design Contradiction:
ProductivityVSObject-affected harmful factors

Solution Approach 1:

The hydrophilic foil cover is segmented by introducing a peripheral edge that extends across the flow channel, dividing the cover into regions that serve different functions: one region maintains wicking performance while the other region allows controlled fluid flow, thus resolving the contradiction between wicking enhancement and flow hindrance

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The peripheral edge of the hydrophilic foil cover acts as an intermediary structure between the absorbing zone and the flow channel. This edge structure mediates the interaction between the cover and fluid flow, allowing the cover to provide wicking support while preventing excessive flow hindrance and contamination risks

Inventive Principle:
Principle #24Intermediary (Mediator)

2Quantity of substance

If the flow channel is narrow to reduce sample volume, then sample volume is reduced, but flow stoppages and mixing inefficiency occur

Engineering Contradiction:
Improvesample volumeVSAvoidflow continuity and mixing efficiency
Core Design Contradiction:
Quantity of substanceVSProductivity

Solution Approach 1:

The flow channel exhibits local quality variations with different sections having different width characteristics. The channel is narrow in the detection zone to reduce sample volume requirements, while broader sections are provided in flow transition zones to maintain flow continuity and mixing efficiency, thus resolving the contradiction between sample volume reduction and flow performance

Inventive Principle:
Principle #3Local quality

3Device complexity

If the assay device uses conventional design without flow control features, then the device complexity is low, but sample wastage and prolonged assay times occur

Engineering Contradiction:
Improvedevice structureVSAvoidsample wastage
Core Design Contradiction:
Device complexityVSLoss of substance

Solution Approach 1:

The assay device incorporates preliminary flow control features including the peripheral edge structure and hydrophilic foil cover configuration that are designed in advance to optimize fluid flow characteristics. These features preliminarily control sample distribution and flow rate, preventing sample wastage and reducing assay time without requiring complex additional components

Inventive Principle:
Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This design reduces sample wastage, enhances mixing and dissolution of detection materials, and improves assay precision by allowing smaller sample volumes with adjustable flow times, while minimizing flow stoppages and contamination risks.

Implementation Method 1

a plurality of projections extending from the substrate surface that are configured to enable capillary flow of an introduced fluid along the fluid flow path

Methodology Applied
Scientific EffectCapillary flow: Capillary Action

Implementation Method 2

a hydrophilic foil or tape cover disposed over the absorbing zone, said cover having a peripheral edge extending across the entrance of the absorbing zone across a flow channel entering the absorbing zone

Methodology Applied
Scientific EffectHydrophilic effect: Hydrophile

Data Source

PatentEP3385713B1Lateral flow assay device
Publication Date: 2023.04.05 ORTHO CLINICAL DIAGNOSTICS INC
  • EP3385713B1 patent drawingFigure 1
  • EP3385713B1 patent drawingFigure 2~3
  • EP3385713B1 patent drawingFigure 4

AI summary

A lateral flow assay device includes a substrate having a top surface, as well as a sample receiving area disposed upon the top surface. At least one fluid flow path extends along the substrate from the sample receiving area, wherein the sample receiving area can be placed in contact with a peripheral reservoir formed at a sample addition area to draw sample therefrom in a controlled manner. The device can further include a reagent area that is designed to promote uniform dissolution of a deposited detection material by a sample moved through the device along the fluid flow path as well, as a flow channel configure to promote mixing of sample and reagent and an absorbing or wicking zone configured to affect various flow characteristics.