Lateral Flow Immunoassay Strip with Fluorescent Detection Zones
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Solution Overview
Problem
Current lateral flow assay devices are limited in their ability to detect multiple analytes with high sensitivity and accuracy, particularly in determining the presence of a first and second analyte in a biological sample, often requiring more advanced methods for quantitative measurement and wider dynamic range.
Innovation Solution
A method involving a test strip with a labeling zone and capture zones, where mobilizable detection reagents conjugated to fluorescent labels bind specifically to analytes, allowing for optical signal detection that decreases or increases with analyte concentration, enabling the simultaneous detection and quantification of multiple analytes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional lateral flow assay devices are used to detect multiple analytes, then the device structure and reagent configuration become complex, but the sensitivity and measurement precision remain insufficient
Solution Approach 1:
The test strip is divided into distinct functional zones: a sample application zone, a detection zone with capture reagents, and a control zone. Each zone contains specifically positioned reagents that perform dedicated functions, allowing multiple analytes to be detected through spatially separated detection regions while maintaining overall device simplicity
Solution Approach 2:
Detection reagents are used as intermediaries that specifically bind to target analytes in the sample. These detection reagents then interact with capture reagents immobilized on the test strip, creating a mediated detection system that enhances sensitivity while keeping the device structure manageable through standardized reagent interactions
2Measurement precision
If conventional lateral flow assay devices are used for quantitative measurement, then the dynamic range is limited, but the device complexity and reagent requirements increase
Solution Approach 1:
The assay utilizes changes in optical parameters (signal intensity, colorimetric properties) of detection reagents upon analyte binding. By measuring these parameter changes at the detection zone, the system achieves quantitative measurement capability with extended dynamic range while maintaining a relatively simple device structure that relies on standard lateral flow architecture
Solution Approach 2:
The test strip design incorporates universal detection mechanisms that can quantify multiple analytes using the same basic detection principles and reagent interactions. The capture reagents and detection reagents are configured to provide multi-functional detection capability, allowing a single device structure to perform accurate quantitative measurements for different analytes without requiring fundamentally different configurations
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances the sensitivity and accuracy of detecting multiple analytes, providing a wider dynamic range and enabling more precise quantitative measurements compared to existing devices.
Implementation Method 1
a first fluorescent label and a second fluorescent label... detecting: (i) a first optical signal from the first fluorescent label... and (ii) a second optical signal from the second fluorescent label
Data Source
AI summary
Disclosed herein are devices, systems, methods and kits for performing immunoassay tests on a sample. The immunoassay devices may be used in conjunction with diagnostic reader systems for obtaining a sensitive read-out of the immunoassay results. The immunoassay devices may be especially suited for the detection of at least a first analyte and a second analyte in a sample. The immunoassay devices and methods may utilize a competitive binding-like assay and a sandwich binding assay to detect analytes in a sample.