Laterally Extended TIMS for High-Capacity Ion Separation
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Solution Overview
Problem
Trapped ion mobility spectrometry (TIMS) analyzers face limitations in storage capacity due to space charge effects, which restrict their ability to analyze low-abundant analytes in complex samples like those in proteomics and metabolomics, despite advancements in ion separation techniques.
Innovation Solution
The development of a laterally extended TIMS (LXTIMS) with an elongate cross-sectional profile and varying electrode structures that generate spatially dependent axial forces, allowing ions to be trapped and separated in an extended volume rather than a line, thereby increasing charge capacity without compromising mobility resolution.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If ions are accumulated at spatially separated positions according to their mobility using higher order fields, then ion separation resolution is improved, but storage capacity is limited due to space charge effects
Solution Approach 1:
The patent transitions from a one-dimensional linear ion trap to a two-dimensional planar trap configuration. The planar trap uses parallel plate electrodes arranged in layers, creating an extended trapping volume in the lateral dimension while maintaining the axial separation by mobility. This dimensional expansion allows many more ions to be stored simultaneously without increasing the charge density to levels where space charge effects degrade mobility resolution.
Solution Approach 2:
The ion trap is segmented into multiple discrete planar trapping regions arranged in series along the axial direction. Each planar trap segment can independently accumulate and store ions, and the segments are connected through drift tubes that maintain the electric field gradient. This segmentation allows the total storage capacity to be distributed across multiple regions, increasing overall capacity while each segment maintains sufficient resolution.
2Quantity of substance
If the trapping region is extended to increase storage capacity, then sensitivity for low abundant analytes is improved, but device complexity increases
Solution Approach 1:
The planar trap structure serves multiple functions simultaneously: it provides ion accumulation, ion storage, and ion separation by mobility all within a single integrated configuration. The parallel plate electrode arrangement creates both the trapping potential well and the electric field gradient needed for mobility separation, eliminating the need for separate components that would increase device complexity.
Solution Approach 2:
The patent changes the geometric parameters of the trapping region from a compact linear arrangement to an extended planar configuration with increased lateral dimensions. By increasing the area of the parallel plates while maintaining a manageable separation distance, the trapping volume is expanded significantly, allowing greater ion capacity without requiring proportionally longer axial dimensions that would complicate the overall device layout.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The LXTIMS significantly enhances storage capacity, enabling the analysis of more low-abundant analytes by trapping ions in a larger volume while maintaining high mobility resolution, thus improving sensitivity in complex sample analysis.
Implementation Method 1
a first force-generator for exerting a first axial force on the ions along the axis, wherein at least one of the first and second axial forces has an effect on the ions that is ion mobility dependent
Implementation Method 2
a second force-generator for exerting a second axial force on the ions along the axis which is counteracting the first axial force
Implementation Method 3
the first and/or the second force generator is configured to vary the magnitude of the first force relative to the second force over time during an elution phase such that the ions are progressively driven to the exit as a function of ion mobility
Data Source
AI summary
The invention provides a trapped ion mobility separator (TIMS) and methods to operate it wherein an ion region of the TIMS, through which ions travel along an axis from an entrance to an exit, has an elongate cross-sectional profile perpendicular to the axis with a long dimension and a short dimension. First and second counteracting forces on the ions along the axis are provided, wherein at least one of the first and second forces has an effect on the ions that is ion mobility dependent, and wherein at least one of the first and second forces varies spatially along the axis such that ions are trapped and separated by ion mobility. Different embodiments provide the first and second forces using different combinations of gas flow and electric field potential, and employ various electrode structures that provide the system with different advantageous characteristics.


