Latex Immunoassay Interference Reduction via Silicone Blocking
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Solution Overview
Problem
The latex immunoagglutination assay (LTIA) method is interfered with by water-soluble silicone and surfactants from blood collection tubes, leading to reduced measurement values and nonspecific adsorption, particularly in micro blood collection tubes, where interference is prominent due to higher concentrations of blood collection tube-processing agents.
Innovation Solution
Incorporating polyether-modified silicone oil into the latex immunoagglutination reaction, which reduces interference from water-soluble silicone and surfactants like BRIJ® 35, TWEEN® 20, and TRITON® X-100 by acting as a blocking agent, thereby stabilizing the antibody-supporting latex particles and maintaining measurement accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If blood collection tube-processing agents (water-soluble silicone/surfactants) are applied to prevent blood clotting and sticking, then blood collection efficiency is improved, but measurement accuracy in LTIA method deteriorates due to interference
Solution Approach 1:
The invention introduces a blocking protein as an intermediary substance that mediates between the surfactant-containing sample and the antibody-latex particles. The blocking protein specifically binds to surfactants and hydrophobic substances, preventing them from interacting with the antibody-latex particles, thus eliminating the interference while preserving the blood collection tube's anticoagulant function
Solution Approach 2:
The invention changes the chemical composition parameter of the reaction system by adding a blocking protein with specific surfactant-binding properties. This parameter change transforms the system from one where surfactants directly interfere with antigen-antibody reactions to one where the blocking protein sequesters surfactants, thereby restoring measurement accuracy
2Quantity of substance
If micro blood collection tubes are used to collect sufficient blood, then blood collection volume is improved, but measurement accuracy deteriorates due to higher concentration of interfering agents
Solution Approach 1:
The blocking protein serves as a mediator that specifically targets and binds to the higher concentrations of surfactants and hydrophobic substances present in samples from micro blood collection tubes, preventing their harmful effects on the LTIA measurement system while allowing accurate measurement of the collected blood volume
3Ease of operation
If surfactants are present in the measurement system, then blood collection tube function is maintained, but antigen-antibody reaction stability deteriorates
Solution Approach 1:
The blocking protein acts as an intermediary that selectively binds to surfactants and hydrophobic substances, creating a protective barrier that prevents these substances from disrupting the antigen-antibody reaction. This allows the blood collection tube to maintain its anticoagulant function while the blocking protein preserves reaction stability
Solution Approach 2:
The invention converts the harmful effect of surfactants into a beneficial situation by having the blocking protein specifically bind to them. The surfactants, which would normally disrupt the measurement, are now sequestered by the blocking protein, preventing interference and stabilizing the antigen-antibody reaction
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for accurate LTIA measurements even with samples collected from micro blood collection tubes, minimizing the impact of water-soluble silicone and surfactant interference, ensuring reliable results by maintaining the integrity of the antigen-antibody reaction.
Implementation Method 1
Incorporating polyether-modified silicone oil into the latex immunoagglutination reaction, which reduces interference from water-soluble silicone and surfactants like BRIJ® 35, TWEEN® 20, and TRITON® X-100 by acting as a blocking agent
Implementation Method 2
a latex immunoagglutination reaction is performed in the presence of a silicone compound in a latex agglutination immunoassay using latex particles supporting a substance having a high affinity for the analyte or supporting the analyte
Implementation Method 3
detect a degree of agglutination (turbidity) of latex particles generated due to binding of an antigen, i.e., the target component, and the antibody-supporting latex particles
Data Source
AI summary
An object of the present invention is to provide a method of reducing interference to a measurement system from water-soluble silicone and/or surfactant contaminating the measurement system in a latex agglutination immunoassay. The execution of a latex immunoagglutination reaction in the presence of a silicone compound can reduce the interference to the measurement system from a component derived from a micro blood-collection tube (water-soluble silicone) and/or surfactant mixed from outside of the measurement system.