Layered Ti-Cu MOFs for One-Step Biological Sample Purification
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing metal organic frameworks (MOFs) are inefficient in adsorbing complex matrices in clinical biological samples, limiting their use in the rapid separation and purification of phenothiazine drugs and metabolites, and their controllable synthesis is challenging.
Innovation Solution
The development of layered super-hydrophilic Ti—Cu-MOFs through a solvothermal reaction process using copper and titanium sources with aromatic amine ligands and stabilizers, resulting in a lamellar structure with hydroxyl groups for enhanced adsorption capacity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional MOFs are used as adsorbents to adsorb target analytes, then the detection of phenothiazine drugs can be achieved, but the complex matrix in the sample cannot be effectively removed
Solution Approach 1:
The patent utilizes the porous structure of MOF materials to achieve effective adsorption. The layered structure with adjustable pore sizes allows selective adsorption of complex matrices while permitting target analytes to pass through, thereby removing matrix interference without compromising detection sensitivity
Solution Approach 2:
The patent employs composite MOF materials combining different metal centers and organic linkers to create a material with enhanced adsorption capabilities. This composite structure enables simultaneous adsorption of complex matrices and maintenance of target analyte detection, resolving the contradiction between matrix removal and detection sensitivity
2Manufacturing precision
If MOFs with controllable morphology and structure are developed, then the separation and purification performance is improved, but the synthesis difficulty increases
Solution Approach 1:
The patent systematically varies synthesis parameters including metal ion ratios, linker types, solvent compositions, temperature, and pH to control MOF morphology and structure. By establishing parameter-performance relationships, the patent achieves controllable synthesis of MOFs with optimized separation and purification performance
Solution Approach 2:
The patent employs preliminary characterization and optimization of synthesis conditions before large-scale preparation. By pre-determining optimal parameters through systematic studies, the patent simplifies subsequent manufacturing while maintaining high separation and purification performance
3Reliability
If multi-step separation and purification methods are used, then the purification effect is improved, but the analysis time increases
Solution Approach 1:
The patent combines multiple separation and purification functions into a single MOF material. The layered structure with specific adsorption sites enables simultaneous removal of different matrix components and concentration of target analytes in one step, achieving multi-functionality that replaces traditional multi-step procedures and reduces analysis time
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The Ti—Cu-MOFs provide a one-step separation and purification method for phenothiazine drugs and metabolites, improving pretreatment efficiency and reducing analysis time, with high adsorption capacity for complex matrices in biological samples.
Implementation Method 1
the complex matrix in sample extract can be adsorbed by an adsorption filler to realize one-step separation and purification of the target analyte
Implementation Method 2
The development of layered super-hydrophilic Ti—Cu-MOFs through a solvothermal reaction process using copper and titanium sources with aromatic amine ligands and stabilizers
Data Source
AI summary
Layered super-hydrophilic Ti—Cu-MOFs and a preparation method and use thereof provided, which relates to the technical field of composite materials. According to the present application, copper ions and titanium ions are used as bimetallic centers to have a coordination reaction with rigid aromatic amine ligands, and layered super-hydrophilic Ti—Cu-MOFs with a lamellar structure is efficiently synthesized. Compared with the prior technology, the preparation method of the present application is simple, and the morphology and structure are controllable; the surface of the prepared layered super-hydrophilic Ti—Cu-MOFs are rich in hydroxyl groups, and have high adsorption capacity for phospholipid co-extracts in clinical biological samples, and thus can be used for the analysis of phenothiazine drugs and metabolites thereof in biological samples and realize one-step separation and purification, thus greatly improving the pretreatment efficiency of clinical biological samples and shortening the analysis time.