Lactobacillus casei LC89 Cultivation for High SCFA Yield in IBD
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Solution Overview
Problem
Existing probiotics do not produce short-chain fatty acids in high enough yields to effectively alleviate inflammatory bowel diseases, which are chronic intestinal inflammatory diseases with increasing incidence and severe symptoms such as abdominal pain, diarrhea, and malnutrition.
Innovation Solution
A high-yield Lactobacillus casei strain, named LC89, is cultivated under specific conditions to produce short-chain fatty acids, which are then formulated into a probiotic powder for oral administration to treat inflammatory bowel diseases.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional probiotic strains are used, then the intestinal microbial balance can be regulated, but the yield of short-chain fatty acids is insufficient to effectively alleviate inflammatory bowel diseases
Solution Approach 1:
The patent identifies and selects specific strain parameters (Lactobacillus casei LC89) with optimized characteristics for high short-chain fatty acid production. The strain is characterized by specific physiological parameters including high SCFA yield, appropriate growth temperature (25-45°C), and pH tolerance (5.0-7.0), which collectively enable effective treatment of inflammatory bowel diseases
2Reliability
If high-yield short-chain fatty acid producing strains are developed, then the effectiveness in treating inflammatory bowel diseases improves, but the complexity of strain characterization and cultivation increases
Solution Approach 1:
The patent establishes specific cultivation parameters including temperature (25-45°C), pH (5.0-7.0), and culture medium composition (containing glucose, beef extract powder, peptone, yeast extract paste, and various salts) to optimize short-chain fatty acid production by Lactobacillus casei LC89, simplifying the cultivation process while maintaining high effectiveness
Solution Approach 2:
The patent employs liquid chromatography for detecting short-chain fatty acid content, replacing complex manual analysis methods with a standardized instrumental approach that simplifies the detection process while ensuring accurate measurement of SCFA yield
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The Lactobacillus casei LC89 strain produces short-chain fatty acids at higher yields than conventional strains, effectively alleviating symptoms of inflammatory bowel diseases in animal and human trials, including reducing abdominal pain, diarrhea, and improving appetite.
Implementation Method 1
SCFA is mainly derived from the fermentation of carbohydrates by anaerobic bacteria in the colon
Implementation Method 2
Short-chain fatty acids (SCFAs), also known as volatile fatty acids, are organic fatty acids composed of 1-6 carbon atoms
Data Source
AI summary
Provided is a Lactobacillus casei producing short-chain fatty acids in a high yield, a culture method therefor and an application thereof. Lactobacillus casei LC89 capable of producing short-chain fatty acids in a high yield is preserved in the China General Microbiological Culture Collection Center on 5 Mar. 2018, with the accession number of CGMCC NO. 15409 and a classification name of Lactobacillus casei. Provided is a Lactobacillus casei producing short-chain fatty acids in a high yield, which is superior to the commercial strain, Lactobacillus rhamnosus GG (LGG). Also provided are a cultivation method therefor and an application thereof in the treatment of host inflammatory bowel diseases to achieve functions of supplementing probiotics to regulate the intestinal tract and alleviating host inflammatory bowel diseases.