LCD Array Quantitative Phase Imaging Microscope
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Solution Overview
Problem
Conventional phase-contrast microscopy lacks the capability for quantitative phase measurement, and existing methods like holographic interference and coherent diffractive imaging require precise optical control, moving parts, or high dynamic range recordings, making them impractical for routine use in optical microscopes.
Innovation Solution
An optical microscope apparatus incorporating a liquid crystal display (LCD) array and lens elements that generates scattered radiation patterns without the need for precise optical pathways or moving parts, allowing for the detection of intensity patterns to construct image data without large dynamic range issues.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If holographic interference methods are used for quantitative phase measurement, then phase information can be obtained, but the system requires exacting requirements on path lengths and optical properties, making it unavailable as a simple add-on to existing microscopes
Solution Approach 1:
The patent replaces mechanical/optical moving parts with an LCD array that can be integrated into the microscope's existing optical pathway. The LCD array electronically modulates light transmission to create multiple scattered radiation patterns without requiring physical movement of optical components, thereby reducing mechanical complexity while maintaining quantitative phase measurement capability
Solution Approach 2:
The LCD array serves multiple functions: it acts as a spatial light modulator to generate scattered radiation patterns, functions as a programmable aperture, and can be integrated into existing microscope objectives without requiring a complete system redesign, making the solution universally applicable to various microscope configurations
2Area of stationary object
If coherent diffractive imaging (CDI) is used, then large working distances and large field of view are achieved, but high dynamic range recordings are required and the imaging time is relatively long
Solution Approach 1:
The patent uses periodic modulation of the LCD array to generate a series of scattered radiation patterns that are captured sequentially. By systematically varying the LCD pixel states across multiple frames, the system reconstructs quantitative phase information from multiple measurements, achieving both large field of view and reduced imaging time compared to traditional CDI methods
Solution Approach 2:
The LCD array pre-configures specific pixel patterns before each exposure, allowing the system to capture multiple scattered radiation patterns in a controlled sequence. This preliminary configuration enables efficient data collection that reduces total imaging time while maintaining the large field of view capability
3Ease of operation
If LCD array with pixel patterns is used to generate scattered radiation patterns, then precise optical pathway control and moving parts are eliminated, but the system must manage the complexity of pattern generation and data reconstruction
Solution Approach 1:
The LCD array is controlled by a computer that automatically generates the required pixel patterns, captures the scattered radiation patterns, and performs the iterative reconstruction algorithm. The system self-manages the complex coordination between pattern generation and data processing without requiring manual intervention, making the operation simple despite the underlying complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the generation of image data for constructing images of target objects with reduced complexity and dynamic range, facilitating quantitative phase imaging without the need for precise optical control or additional moving parts, thus improving the efficiency and practicality of phase measurement in microscopy.
Implementation Method 1
detecting an intensity of radiation scattered by the target object with a liquid crystal display (LCD) array, providing a first pixel pattern
Implementation Method 2
an objective lens arrangement which incorporates an LCD device therein. By selecting a pattern of on-off pixels of the LCD distinct scattering patterns can be detected
Data Source
AI summary
A method and apparatus are disclosed for generating a plurality of scattered radiation patterns at an image plane of an optical microscope. The apparatus includes at least one lens element, a liquid crystal display (LCD) array, and a housing comprising a body portion supporting the LCD array and lens element in a predetermined spaced apart relationship. The LCD array comprises a plurality of pixel elements arranged in a grid layout, said array being connectable to a processing element adapted to selectively control a transmittance of each pixel in the grid layout.