LCOS-SLM Light Stimulation Device with Constant Irradiation Power
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Solution Overview
Problem
Conventional LCOS-SLM devices require cumbersome operations to adjust the number of stimulated spots and irradiation power, as the power of stimulation light for each spot decreases inversely with the number of spots, necessitating correlated changes in settings.
Innovation Solution
A light stimulation device with a phase modulating section, light-amount adjusting section, spot specifying section, and light-amount control section that allows for easy adjustment of the number of spots or spot area while maintaining constant irradiation power by controlling the light amount and pupil modulation pattern, ensuring consistent light distribution across the specimen.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Area of stationary object
If the number of stimulated spots is increased using an LCOS device, then the coverage area of light stimulation is improved, but the irradiation power for each spot decreases in inverse proportion
Solution Approach 1:
The system performs preliminary calculation of the optimal light amount based on the number of spots and spot area before actual light stimulation. The light amount control section pre-determines the required light amount to maintain constant irradiation power, avoiding the need for manual adjustment during the experiment.
Solution Approach 2:
The system implements a feedback mechanism where the light amount control section continuously monitors the number of spots and spot area, and automatically adjusts the light amount to the optimal value to maintain constant irradiation power for each spot.
2Stability of the object's composition
If the irradiation power for each spot is maintained constant while changing the number of spots, then the consistency of light stimulation is improved, but the operation complexity increases due to correlated adjustments
Solution Approach 1:
The system performs self-adjustment by automatically calculating and controlling the light amount based on the number of spots and spot area. The light amount control section enables the system to maintain constant irradiation power without requiring manual intervention, making the system serve itself.
Solution Approach 2:
The system dynamically changes the light amount parameter based on the number of spots and spot area to maintain constant irradiation power. The light amount control section adjusts this parameter automatically, eliminating the need for manual correlated adjustments.
3Measurement precision
If manual adjustment of light amount is required when changing spot configuration, then the precision of irradiation power control is improved, but the time consumption increases
Solution Approach 1:
The system replaces manual mechanical adjustment with automated electronic control. The light amount control section uses electronic signals to adjust the light amount, substituting the manual mechanical adjustment process and thereby reducing time consumption while maintaining precision.
Solution Approach 2:
The system performs preliminary calculation of the optimal light amount before actual adjustment is needed. The light amount control section pre-determines the required light amount based on spot configuration, enabling rapid adjustment without time-consuming manual calculations.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables effortless increase or decrease in the number of stimulated spots or spot area with constant irradiation power, facilitating efficient light stimulation and observation of specimen reactions.
Implementation Method 1
a phase modulating section that is located at a position conjugate to a pupil of the objective lens and that can modulate the phase of light to be made to enter the objective lens, based on a predetermined pupil modulation pattern
Data Source
AI summary
The number of stimulated spots and/or the area of a stimulated spot is easily increased or decreased while keeping the irradiation power for each stimulated spot constant. Provided is a light stimulation device (3) that includes an objective lens (23) that radiates stimulation light emitted from a stimulation laser (11) onto a specimen (S); an LCOS-SLM (17) that is located at a position conjugate to the pupil of the objective lens (23) and that can modulate the phase of stimulation light to be made to enter the objective lens (23) based on a predetermined pupil modulation pattern; an AOM (13) that adjusts the amount of stimulation light to be made to enter the LCOS-SLM (17); a mouse (26) that is used to specify the number of spots and the position of a spot of light to be radiated onto the specimen (S); and a controller main unit (28) that determines the pupil modulation pattern for the LCOS-SLM (17) according to the number of spots and the position of a spot specified by means of the mouse (26) and that controls the AOM (13) such that, when the number of spots specified by means of the mouse (26) is changed, the amount of light to be radiated onto each spot before and after the change in the number of spots becomes constant.